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M9400

Sigma-Aldrich

β-Mannosidase from Helix pomatia

5-30 units/mL, ammonium sulfate suspension, crude extract

Synonyme(s) :

β-D-Mannoside mannohydrolase

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About This Item

Numéro CAS:
9025-43-8
Numéro de classification (Commission des enzymes):
3.2.1.25 (BRENDA, IUBMB)
Numéro MDL:
MFCD00130638
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32

Forme

ammonium sulfate suspension

Qualité

crude extract

Poids mol.

94 kDa

Concentration

5-30 units/mL

Activité étrangère

β-N-acetylglucosaminidase and α-mannosidase <1.0%

Température de stockage

2-8°C

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Description générale

β-Mannosidase is a lysosomal enzyme that belongs to the glycosyl hydrolase family 2.

Application

β-Mannosidase from Helix pomatia has been used:
  • in the digestion of glycans for enzymatic analysis
  • in the treatment of labeled monogalactosyl Manno-oligosaccharides
  • in non-mammalian glycosidase inhibition assays

Actions biochimiques/physiologiques

β-Mannosidase participates in cleaving the β(1–4)-linked mannose located at the nonreducing end of N-glycosylated proteins. It is involved in the polysaccharide degradation pathway. In humans, mutations in the gene are associated with β-mannosidosis, a lysosomal storage disease. Low levels of β-Mannosidase due to mutations also lead to nystagmus, an eye disorder associated with involuntary eye movements.

Définition de l'unité

One unit will hydrolyze 1 μmole of p-nitrophenyl-β-D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.

Forme physique

Suspension in 3.0 M (NH4)2SO4 containing 10 mM sodium acetate, pH approx. 4.0

Inhibiteur

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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P Wongputtisin et al.
Journal of applied microbiology, 113(4), 798-806 (2012-07-14)
To screen and select the Bacillus spp. from Tua-nao of northern Thailand for fermented corticate soybean meal (FCSBM) production. After isolation of Bacillus spp. from Tua-nao was carried out, cellulase, hemicellulases (i.e., β-mannanase and xylanase) and phytase production by isolated
Roneel Prakash et al.
Journal of plant physiology, 169(12), 1125-1133 (2012-06-05)
Cell walls of tomato fruit contain hemicellulosic mannans that may fulfill a structural role. Two populations were purified from cell walls of red ripe tomato tissue and named galactoglucomannan-glucuronoxylan I and II (GGM-GX I and II), respectively. Both polysaccharides not
Pengfu Liu et al.
Applied biochemistry and biotechnology, 167(1), 73-80 (2012-04-25)
A novel biosensor strain (Escherichia coli ALM403) that responded to N-acyl homoserine lactone (AHL) was constructed using a luxR-Plux cassette as a regulatory sequence and β-mannanase as a reporter gene. Dinitrosalicylic acid method was used to detect the response of
Umme Afsari Zahura et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 162(1-3), 24-33 (2012-03-01)
A β-D-mannosidase (EC 3.2.1.25) with a molecular mass of approximately 100 kDa was purified from the digestive fluid of a marine gastropod Aplysia kurodai by ammonium sulfate fractionation followed by column chromatographies on TOYOPEARL Butyl-650 M, TOYOPEARL DEAE-650 M, and
A A Klyosov et al.
Carbohydrate research, 352, 65-69 (2012-03-23)
Statistical modeling was applied for describing structural features of β-(1→4)-D-galactomannans. According to the model suggested theoretical ratios of limiting degrees of locust bean, tara gum and guar gum galactomannan conversions by two β-(1→4)-mannanases of different origin (Myceliophthora thermophila and Trichoderma
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