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M8537

MCDB 131 Medium

Name: MCDB 131 Medium
Brand: SIGMA

With ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonyme(s) :

131 Medium, Medium 131, Molecular, Cellular, and Development Biology 131 medium

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A propos de cet article

UNSPSC Code:

12352207

NACRES:

NA.75

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Propriétés

Nom du produit

MCDB 131 Medium, With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

concentration

11.6 g/L

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

Description

General description

MCDB-131 medium provides a defined and optimally balanced nutritional environment that selectively promotes the growth of specific cell types. MCDB-131 is designed to support clonal growth of human microvascular endothelial cells, including human umbilical vein endothelial cells, when supplemented with 0.7% FBSP, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. MCDB media is made for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP).

Application

MCDB-131 Medium has been used:

as a component of the basal medium to culture bovine retinal endothelial cells[1]
to culture bovine lung microvascular endothelial cells[2]
for the isolation of porcine endocranial endothelial cells and porcine aortic endothelial cells[3]

Preparation Note

Formulated to contain 11.6 grams of powder per liter of medium.

Supplement with 1.18 g/L sodium bicarbonate.

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Cet article	M7403	M6395	M6770
Sigma-Aldrich M8537 MCDB 131 Medium	Sigma-Aldrich M7403 MCDB 153 Medium	Sigma-Aldrich M6395 MCDB 105 Medium	Sigma-Aldrich M6770 MCDB 201 Medium
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form powder	form powder	form powder
components sodium pyruvate: 0.11 g/L phenol red: 0.012 g/L L-glutamine: 1.461 g/L HEPES: no glucose: 1.0 g/L (Dextro)	components NaHCO₃: no L-glutamine: 0.8772 g/L glucose: 1.081 g/L (Dextro) sodium pyruvate: 0.055 g/L phenol red: 0.001242 g/L HEPES: 6.6 g/L (25mM)	components phenol red: 0.001242 g/L glucose: 0.72064 g/L (Dextro) sodium pyruvate: 0.11 g/L HEPES: 5.958 g/L (25mM) L-glutamine: 0.3653 g/L	components HEPES: 7.149 g/L (30mM) glucose: 1.441 g/L (Dextro) phenol red: 0.001242 g/L sodium pyruvate: 0.055 g/L L-glutamine: 0.14615 g/L
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
concentration 11.6 g/L	concentration -	concentration -	concentration -

Classe de stockage

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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