Yes, i is advised to use a white plate with a clear bottom for a luciferase assay. The primary difference between white and black plates lies in their reflective properties. White plates reflect light, maximizing light output signal, which is suitable for luminescent assays. On the other hand, black plates absorb light, reducing background and crosstalk, making them ideal for fluorescent assays. In multiplexed assays, using a white plate will maximize light output signal for the luminescent portion but may lead to higher crosstalk and background for the fluorescent part. Conversely, using a black plate will reduce fluorescent signal crosstalk and background, but will also reduce the luminescence signal by about 1 order of magnitude.
M5686
Greiner high and medium binding 96 well plates
flat bottom clear polystyrene wells (with micro-clear bottom), medium binding surface, non-sterile
Synonyme(s) :
96 multiwell plates, 96 well microplates, 96 well microtiter plates, 96 well strip plates
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About This Item
Code UNSPSC :
41122107
Nomenclature NACRES :
NB.22
Matériaux:
black polystyrene
flat bottom clear polystyrene wells (with micro-clear bottom)
flat bottom clear polystyrene wells (with micro-clear bottom)
Volume maximum:
392 μL
Taille:
96 wells
Volume de travail:
340 μL
Stérilité:
non-sterile
Type de liaison:
medium binding surface
Caractéristiques:
lid: no
skirt (F-bottom)
skirt (F-bottom)
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Matériaux
black polystyrene
flat bottom clear polystyrene wells (with micro-clear bottom)
Stérilité
non-sterile
Caractéristiques
lid: no
skirt (F-bottom)
Conditionnement
case of 40
Fabricant/nom de marque
Greiner 655096
Volume maximum
392 μL
Taille
96 wells
Volume de travail
340 μL
Type de liaison
medium binding surface
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Description générale
FLUOTRAC™ 200 96W Microplate, well working volume 25-340 μL; total well volume 392 μL.
Polystyrene Multiwell plates from Greiner for immunoassay and high throughput screening applications. High binding plates are recommended for ELISAs. High binding denotes a binding capacity of 600 ng/cm2 and scatter of less than 10%. Optimal protein binding is variable and comparative tests between high and medium binding plates is suggested.
Autres remarques
96 well flat (F) μClear® bottom; medium binding microplate, chimney style, no lid, alphanumeric well coding, length: 127.76 mm; width: 85.48 mm; curvature approx. 200 um.
Adéquation
Black plates minimize background fluorescence. FLUOTRAC 200 binds up to 200 ng/cm2. Clear bottoms allow for monitoring thru the plate bottom. Free of detectable DNase/RNase, human DNA and pyrogens.
Informations légales
μClear is a registered trademark of Greiner Bio-One GmbH
FLUOTRAC is a trademark of Greiner Bio-One GmbH
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Protocoles
This assay protocol is suitable for the colorimetric/fluorometric detection of phenylalanine in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Phenylalanine Assay Kit (MAK005). Phenylalanine concentration is determined by a coupled enzyme assay, which results in the deamination of phenylalanine and the production of NADH which reacts with the probe resulting in a fluorometric (λex = 535/λem = 587 nm) product, proportional to the phenylalanine present. The linear range of detection for this assay is between 0.2–1.0 nmole.
Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.
Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.
Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.
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Can this product be used for a luciferase assay?
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