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I1884

Sigma-Aldrich

Insulin-transferrin-sodium selenite media supplement

γ-irradiated, lyophilized powder, BioXtra, suitable for cell culture

Synonyme(s) :

ITS Supplement

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About This Item

Code UNSPSC :
12352205
Nomenclature NACRES :
NA.75

Source biologique

human plasma

Niveau de qualité

200

Stérilité

γ-irradiated

Gamme de produits

BioXtra

Forme

lyophilized powder

Technique(s)

cell culture | mammalian: suitable

Numéro d'accès UniProt

P01308

Conditions d'expédition

ambient

Température de stockage

−20°C

Informations sur le gène

human ... INS(3630)

Description générale

Insulin-transferrin-sodium selenite media supplement supports in vitro growth and maturation of follicles from oocyte and is routinely used in in vitro fertilization methods.
Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine.

INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.

TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.

SELENIUM is an essential trace element normally provided by Serum.

Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.

Application

Insulin-transferrin-sodium selenite media supplement has been used as:
  • medium 199 (M199) media supplement for cardiac myocytes culture
  • Roswell Park Memorial Institute (RPMI) 1640 medium component for pluripotent stem cell (iPSC) culture
  • Dulbecco′s Modified Eagle Medium (DMEM) supplement in ID8 ovarian carcinoma cells

Autres remarques

Each vial contains: ≥21 mg recombinant human insulin; ≥19 mg human transferrin (substantially iron free), and 25 μg sodium selenite.
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Quantité

Each vial sufficient to prepare 5 liters of medium.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

TnI Structural Interface with the N-Terminal Lobe of TnC as a Determinant of Cardiac Contractility
Vetter AD, et al.
Biophysical Journal, 114(7), 1646-1656 (2018)
NFAT5-regulated macrophage polarization supports the proinflammatory function of macrophages and T lymphocytes
Tellechea M, et al.
Journal of immunology (Baltimore, Md. : 1950), 200(1), 305-315 (2018)
The effects of different concentrations of sodium selenite on the in vitro maturation of preantral follicles in serum-free and serum supplemented media
Abedelahi A, et al.
Journal of Assisted Reproduction and Genetics, 25(9-10), 483-488 (2008)
Bu-Er Wang et al.
PloS one, 3(5), e2186-e2186 (2008-05-15)
Although Wnt signaling has been shown to be important for embryonic morphogenesis and cancer pathogenesis of several tissues, its role in prostatic development and tumorigenesis is not well understood. Here we show that Wnt signaling regulated prostatic epithelial branching morphogenesis
Y Zhang et al.
Animal : an international journal of animal bioscience, 1-9 (2018-10-04)
Research on the causes of sheep death in sea voyages from Australia to the Middle East is limited, in particular little is known about the influence of climatic factors. Mortality data from 417 shipments of sheep exported over an 11-year
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Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

Transferrin in Cell Culture

Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

Transferrin in Cell Culture

Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

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