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H6875

Sigma-Aldrich

Hippuryl-L-phenylalanine

Synonyme(s) :

Hippuryl-Phe, N-Benzoyl-Gly-Phe

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About This Item

Formule empirique (notation de Hill):
C18H18N2O4
Numéro CAS:
744-59-2
Poids moléculaire :
326.35
Numéro Beilstein :
2167818
Numéro CE :
212-016-7
Numéro MDL:
MFCD00037265
Code UNSPSC :
12352204
ID de substance PubChem :
24895770
Nomenclature NACRES :
NA.32

Pureté

≥98% (TLC)

Forme

powder

Solubilité

acetic acid: 50 mg/mL, clear, colorless

Température de stockage

−20°C

Chaîne SMILES 

OC(=O)[C@H](Cc1ccccc1)NC(=O)CNC(=O)c2ccccc2

InChI

1S/C18H18N2O4/c21-16(12-19-17(22)14-9-5-2-6-10-14)20-15(18(23)24)11-13-7-3-1-4-8-13/h1-10,15H,11-12H2,(H,19,22)(H,20,21)(H,23,24)/t15-/m0/s1

Clé InChI

CCLJGZGVIQBNDH-HNNXBMFYSA-N

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Application

Hippuryl-L-phenylalanine has been used as a substrate for screening carboxypeptidase activity in Trogoderma granarium, Bactrocera oleae Gmelin and Apodiphus amygdali.

Actions biochimiques/physiologiques

Hippuryl-L-phenylalanine is a substrate for carboxypeptidase A enzyme.

Substrats

Substrate for carboxypeptidase A

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Product stabilization of the two forms of carboxypeptidase A from buffalo pancreas
Sud M and Dua RD
Journal of Biosciences, 9(1-2), 83-90 (1985)
J H Cho et al.
Biochemistry, 40(34), 10197-10203 (2001-08-22)
We have investigated the function of Tyr248 using bovine wild-type CPA and its Y248F and Y248A mutants to find that the K(M) values were increased by 4.5-11-fold and the k(cat) values were reduced by 4.5-10.7-fold by the replacement of Tyr248
P W Tardioli et al.
Biotechnology progress, 19(2), 565-574 (2003-04-05)
This paper presents stable carboxypeptidase A (CPA)-glyoxyl derivatives, to be used in the controlled hydrolysis of proteins. They were produced after immobilizing-stabilizing CPA on cross-linked 6% agarose beads, activated with low and high concentrations of aldehyde groups, and different immobilization
Digestion and protein metabolism of Trogoderma granarium (Coleoptera: Dermestidae) fed on different barley varieties
Mardani-Talaee M, et al.
Journal of Stored Products Research, 73(1-2), 37-41 (2017)
M E Sander et al.
Biochemical and biophysical research communications, 132(2), 681-687 (1985-10-30)
Carboxypeptidase A was incubated at -60 degrees C with an excess of O-(trans-p-chlorocinnamoyl)-L-phenyllactate, O-(hippuryl)-glycolate or N-(hippuryl)-L-phenylalanine. After rapid denaturation with trichloracetic acid the precipitated protein was reduced with [3H]NaCNBH3. 3H Labeled enzyme was isolated by gel chromatography on Sephadex G-25.
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Protocoles

Enzymatic Assay: Carboxypeptidase

Carboxypeptidase A activity measured via continuous spectrophotometric rate determination assay with hippuryl-L-phenylalanine substrate.

Enzymatic Assay: Carboxypeptidase

Carboxypeptidase A activity measured via continuous spectrophotometric rate determination assay with hippuryl-L-phenylalanine substrate.

Enzymatic Assay: Carboxypeptidase

Carboxypeptidase A activity measured via continuous spectrophotometric rate determination assay with hippuryl-L-phenylalanine substrate.

Enzymatic Assay: Carboxypeptidase

Carboxypeptidase A activity measured via continuous spectrophotometric rate determination assay with hippuryl-L-phenylalanine substrate.

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