G6008

β-Galactosidase from Escherichia coli

Grade VI, lyophilized powder, ≥250 units/mg protein

• Synonyme(s) : β-D-Galactoside galactohydrolase, Lactase
• Numéro CAS: 9031-11-2
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-864-1
• MDL number: MFCD00155434
• Numéro CE : 3.2.1.23 (BRENDA, IUBMB)
• Specific activity: ≥250 units/mg protein
• Biological source: Escherichia coli

Propriétés

• biological source: Escherichia coli
• Quality Segment: 200
• type: Grade VI
• form: lyophilized powder
• specific activity: ≥250 units/mg protein
• mol wt: 465 kDa
• does not contain: BSA as extender
• composition: Protein, ≥50% biuret
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Poids moléculaire du tétramère : 465 kDa (chaque sous-unité ayant 116,3 kDa)

β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 Da each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.

Application

β-Galactosidase is conjugated to an antibody that specifically recognizes a target molecule (enzyme immunoassay or EIA). β-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter.

Biochem/physiol Actions

βLa β-galactosidase clive le lactose en ses constituants monosaccharides, le glucose et le galactose. Elle catalyse également la transglycosylation du glucose en allolactose, inducteur de la β-galactosidase, dans une boucle de rétroaction.

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Physical form

Partially purified; contains Tris buffer salts, magnesium chloride, DL-dithiothreitol and 2-mercaptoethanol

Other Notes

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Informations sur la sécurité

• wgk: WGK 3
• Classe de stockage: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)