F4042
Monoclonal ANTI-FLAG® M5 antibody produced in mouse
clone M5, purified immunoglobulin, buffered aqueous solution
Synonyme(s) :
Anti-ddddk, Anti-dykddddk
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A propos de cet article
Conjugate:
unconjugated
Clone:
M5, monoclonal
Application:
WB CL
Citations:
186
Service technique
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Laissez-nous vous aiderbiological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M5, monoclonal
form
buffered aqueous solution
purified by
using Protein A
species reactivity
all
concentration
2-5 mg/mL
technique(s)
western blot (chemiluminescent): 10 μg/mL
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
General description
Monoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.
Method of purification - Protein A
Method of purification - Protein A
Immunogen
FLAG; peptide sequence DYKDDDDK
Application
Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
Monoclonal ANTI-FLAG® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.
Physical form
Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)
Preparation Note
Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.
Other Notes
Antibody is not calcium dependent.
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Classe de stockage
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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