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Key Documents

E3406

Sigma-Aldrich

Anti-EB1 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-APC binding protein EB1, Anti-End-Binding-1, Anti-Microtuble-associated protein RP/EB family member 1

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 35 kDa

Espèces réactives

rat, mouse, bovine, pig, canine, human

Concentration

~1.5 mg/mL

Technique(s)

immunoprecipitation (IP): 10-20 μg using cell lysate of the human epitheloid carcinoma HeLa cell line
indirect immunofluorescence: 1-2 μg/mL using rat fibroblast Rat1 cells
indirect immunofluorescence: suitable
western blot: 0.5-1 μg/mL using cell lysate of the rat fibroblast cell line Rat1 or Rat 2

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

Description générale

Microtubule-associated protein RP/EB family member 1 is a protein encoded by the MAPRE1 gene in humans. EB1 (End-Binding-1) proteins belong to a distinct family of accessory proteins, termed “plus-end tracking proteins” or +TIPs, that bind to the plus-ends of microtubules. All EB1 family proteins have a highly conserved N-terminal region that contains a calponin homology (CH) domain and an EB1-like C-terminal motif, which is unique to EB1 family proteins.

Immunogène

synthetic peptide corresponding to amino acids 252-268 located at the C-terminus of human EB1, conjugated to KLH. This sequence is identical in several species (mouse, rat, dog, bovine, and porcine EB1), highly conserved (single amino acid substitution) in chicken EB1, and has limited homology to human EB2 (35% identity) and human EB3 (53% identity).

Application

Anti-EB1 antibody produced in rabbit has been used in
  • immunofluorescence
  • western blotting
  • immunocytochemistry
  • immunoprecipitation

Actions biochimiques/physiologiques

End-Binding-1 (EB1) associates with components of the dynactin complex and cytoplasmic dynein intermediate chain.
The C-terminal domain of End-binding proteins (EBs) is important for its autonomous microtubule tip tracking, regulation of microtubule dynamics, chromosome segregation and recruitment of numerous partners to microtubule ends. EB1 proteins bind to microtubule ends and act with the adenomatous polyposis coli (APC) tumor suppressor to regulate the microtubule filament system. It is a potential oncogene and plays an important role during early event in colon carcinogenesis, colorectal cancer (CRC). EB1 acts as a potential biomarker and therapeutic target in CRC.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

EB1 contributes to proper front-to-back polarity in neutrophil-like HL-60 cells
Samereier M, et al.
European Journal of Cell Biology, 96(2), 143-153 (2017)
EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability
Tirnauer JS and Bierer BE
The Journal of cell biology, 149(4), 761-766 (2000)
Dominique Seetapun et al.
Current biology : CB, 20(11), 979-988 (2010-05-25)
Breaking cell symmetry, known as polarization, requires dynamic reorganization of microtubules (MTs) and is essential to many cellular processes, including axon formation in neurons. A critical step in polarization is believed to be the "selective stabilization" of MTs, which hypothesizes
Cell-length-dependent microtubule accumulation during polarization
Seetapun D and Odde DJ
Current Biology, 20(11), 979-988 (2010)
Microtubule plus-end tracking proteins in neuronal development
van de Willige D, et al.
Cellular and Molecular Life Sciences, 73(10), 2053-2077 (2016)

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