D4764

Deoxyribonucleic acid from calf thymus

Genomic, unsheared

• Synonyme(s) : double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
• Numéro CAS: 91080-16-9
• UNSPSC Code: 41106310
• eCl@ss: 32160414
• EC Number: 293-507-3
• NACRES: NA.52
• MDL number: MFCD00151698
• Biological source: bovine (calf) thymus
• Form: lyophilized powder
• Storage temp.: 2-8°C

Propriétés

• biological source: bovine (calf) thymus
• Quality Segment: 200
• grade: Molecular Biology
• form: lyophilized powder
• mol wt: >20 kb
• storage temp.: 2-8°C

Description

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.

Biochem/physiol Actions

42% GC; T_m = 87 °C.
% GC is the percentage of G/C base pairs. T_m is the temperature at the midpoint of the thermal denaturation profile.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Preparation Note

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A₂₆₀ units per mL. ~20 A₂₆₀ units per mg DNA.

Informations sur la sécurité

• Classe de stockage: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves