D4522

Deoxyribonucleic acid from calf thymus

Type XV, Activated, lyophilized powder

• Synonyme(s) : Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
• Numéro CAS: 91080-16-9
• UNSPSC Code: 41106310
• eCl@ss: 32160414
• EC Number: 293-507-3
• NACRES: NA.52
• MDL number: MFCD00151698
• Biological source: bovine pancreas
• Form: lyophilized powder
• Solubility: water: slightly soluble
• Storage temp.: −20°C

Propriétés

• biological source: bovine pancreas
• Quality Segment: 200
• grade: Molecular Biology
• type: Type XV
• form: lyophilized powder
• solubility: water: slightly soluble
• storage temp.: −20°C

Description

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Preparation Note

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

One mg DNA is approx. 20 A₂₆₀ units.

Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves