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CS0009

Sigma-Aldrich

Superoxide Dismutase, SOD, Activity Assay Kit

sufficient for 500 colorimetric tests

Synonyme(s) :

CU/ZN-SOD, SOD, SOD1, Superoxide Dismutase, Superoxide Dismutase 1 bovine, Superoxide: superoxide oxidoreductase

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.54
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Niveau de qualité

200

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Superoxide dismutase (SOD) is part of a family of metal ion-containing enzymes (SODs) that are found in most organisms, from bacteria to mammals. SODs catalyze the dismutation of superoxide (generated by aerobic respiration) to molecular oxygen and peroxide. Superoxide dismutase form the front line of defence against reactive oxygen species (ROS)-mediated injury and harbor anti-inflammatory activities. The Superoxide Dismutase (SOD) Activity Assay Kit provides a simple and sensitive procedure for measuring SOD enzymatic activity in various sample types. SOD activity is determined by measuring the decrease in superoxide anions (generated by the enzyme xanthine oxidase [XO]). Since superoxide anions interact with the provided WST dye, yielding color at 450 nm, the decrease in the color signal is proportional to SOD inhibition activity. The kit allows the determination of SOD activity in percent inhibition, or in units of activity using the provided SOD Enzyme as a standard. The provided SOD Enzyme can also be used to screen for SOD inhibitors.
The kit’s lowest limit of detection is 0.3 SOD units/mL. This kit can be used to measure SOD activity in biological samples such as serum, plasma and erythrocytes, tissue homogenates and cell lysates.
Components This kit contains sufficient reagents for 500 colorimetric tests.

Our economical Superoxide Dismutase (SOD) activity assay kit also saves your time and efforts:
  • Highly sensitive, simple and high-throughput assay for measuring SOD activity in a variety of biological samples (serum, plasma,erythrocytes, tissue homogenates and cell lysates)
  • Allows measurement of SOD activity both in percentage of inhibition and also by measuring the activity in enzymatic units using a calibration curve curve.
  • Enables inhibitor screen using our SOD enzymes which is provided as a standard.
  • User friendly: No need to weigh or calculate needed amounts of reagents on your own
  • Supportivecalculator (Click here to download a calculator excel file):will calculate the reagents needed according to your experimental needs, andanalyses your results based on your experimental data!
  • Quick instruction bench card - to assure your experimental success
  • More experiments in one kit - contains sufficient reagents for 500 colorimetric tests.
  • Limit of detection: 0.3 SOD units/mL
  • Detection time: only 30 min
  • Detection can be done at room temperature.

Caractéristiques et avantages

  • Quantifying Superoxide Dismutase , SOD activity in biological samples
  • Screening potential inhibitors for Superoxide Dismutase, SOD, activity

Adéquation

This kit can be used to measure SOD activity in biological samples such as serum, plasma and erythrocytes, tissue homogenates and cell lysates.

Code de la classe de stockage

10 - Combustible liquids

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Certificats d'analyse (COA)

Lot/Batch Number
0000395310
0000324105
0000324104
0000324104
0000324105

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Dewa Ketut Meles et al.
Veterinary medicine international, 2022, 3685686-3685686 (2022-03-04)
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a persistent organic pollutant that induces overproduction of reactive oxygen species (ROS). Studies on avoiding the adverse effects of dioxin pollution exposure are needed in all aspects, including reproductive health. This study aimed to determine the effect
M Ardourel et al.
Experimental eye research, 225, 109282-109282 (2022-10-21)
Aging is a multifactorial process that affects the entire organism by cumulative alterations. Visual function impairments that go along with aging are commonly observed, causing lower visual acuity, lower contrast sensitivity, and impaired dark adaptation. Electroretinogram analysis revealed that the
Alessia Remigante et al.
Frontiers in physiology, 14, 1225552-1225552 (2023-07-17)
Introduction: Aging is a process characterised by a decline in physiological functions. Reactive species play a crucial role in the aging rate. Due to the close relationship between aging and oxidative stress, functional foods rich in phytochemicals are excellent candidates
Masaki Kimura et al.
Cell, 185(22), 4216-4232 (2022-10-15)
Genotype-phenotype associations for common diseases are often compounded by pleiotropy and metabolic state. Here, we devised a pooled human organoid-panel of steatohepatitis to investigate the impact of metabolic status on genotype-phenotype association. En masse population-based phenotypic analysis under insulin insensitive

Questions

1–5 of 5 Questions  

  1. Can we use UV-Vis Spectrophotometer instead of Plate-reader?

    1 answer

    1. This kit is designed to be used with a 96-well plate reader.

      Helpful?

  2. will this work to evaluate the SOD in seminal plasma of goat semen

    1 answer

    1. This kit may work in the stated application. However, this will have to be determined empirically. This kit can be used to measure SOD activity in biological samples such as serum, plasma and erythrocytes, and tissue homogenates and cell lysates.

      Helpful?

  3. Can you confirm if the buffer that comes with the kit contains Manganese?

    1 answer

    1. The buffer provided with Product No. CS0009, Superoxide Dismutase, SOD, Activity Assay Kit does not contain manganese.

      Helpful?

  4. Is this product only compatible with SOD1, or is it also compatible with SOD2 when using our own protein?

    1 answer

    1. The Product No. CS0009, Superoxide Dismutase, SOD, Activity Assay Kit can determine the activity of any of the SOD enzyme isoforms, including SOD1 & SOD2.

      Helpful?

  5. How much serum sample volume needed for this assay?

    1 answer

    1. All assays (samples, standards, controls and blank) require 20 μL of sample for each reaction (well). Therefore, bring the sample volume to 20 μL. When required, samples should be diluted in Dilution Buffer. Kindly review the Product Information Sheet at this link for further details: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/100/209/cs0009bul-ms.pdf

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