A7r5 Cell Line from rat

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86050803, rat muscle, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• Source biologique: rat muscle
• Description: Rat BDIX smooth muscle embryonic aorta
• Conditionnement: tube of 5 μg 86050803-DNA-5UG; pkg of vial of cells 86050803-1VL
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Muscle-type isoenzymes
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat BDIX smooth muscle embryonic aorta

Description de la lignée cellulaire

Flat ribbon-like cells differentiating to parallel arrays of spindle shaped cells. Derived from the thoracic aorta of an embryonic BDIX rat. As cells achieve the stationary phase there is an increase in myokinase and creatine phosphokinase (CPK) activity. After cessation of cell division a muscle type CPK isoenzyme is synthesised.

Application

A7r5 Cell Line from rat has been used to study the effect of angiotensin II on vascular oxidative stress. It has been used in PLA2 inhibitory study to understand the role of PLA2 on formation of lipid droplets.

Biochemical and biophysical studies

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. After resuscitation cells may take up to 7 days before a monolayer forms and growth can be very slow in general. Cells also grow in clumps.

Autres remarques

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