F9

85061803, mouse embryo, Not specified

• Code UNSPSC : 41106514

Propriétés

• product name: F9, 85061803
• Source biologique: mouse embryo
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Not specified
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse 129 embryo teratocarcinoma

Description de la lignée cellulaire

Derived from testicular carcinoma. F9 undergo very limited differentiation under normal conditions but can be induced to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cAMP.

Application

Differentiation studies

Milieu de culture

DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum (FBS). Pre-soak flasks in 0.1% gelatin for over 2 hours and wash with PBS.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂, 37°C. Upon resuscitation, cells shed large quantities of debris, leave for 2 days before subculture. Please note: Culture v

Autres remarques

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