MCC13 cell line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 10092302, human skin, Large, low contrast cells

• Synonyme(s) : Human MCC13 Line, MCC13 Human Cells
• UNSPSC Code: 41106514
• Biological source: human skin
• Relevant disease(s): cancer
• Growth mode: Not specified
• Karyotype: Not specified
• Morphology: Large, low contrast cells
• Manufacturer/tradename: ECACC

Propriétés

• biological source: human skin
• description: Merkel carcinoma from human skin
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 10092302-DNA-5UG, pkg of vial of cells 10092302-1VL
• growth mode: Not specified
• karyotype: Not specified
• morphology: Large, low contrast cells
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Biochem/physiol Actions

Human Merkel carcinoma

Merkel cell carcinoma cell line. Cell Bank Australia reference number: CBA1338

Preparation Note

Optimal split ratio 1:8 (seeding density 0.8 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

RPMI 1640 (with 2mM L-Glutamine+25mM HEPES) + 15% FCS

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Helen Leonard - Queensland Institute of Medical Research, Australia

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.