Mero-25

9100102, human lung, Mixed epithelial and spindle shaped

• Code UNSPSC : 41106514

Propriétés

• product name: Mero-25, 09100102
• Source biologique: human lung
• Mode de croissance: Adherent
• Caryotype: Number of chromosomes 127-140
• Morphologie: Mixed epithelial and spindle shaped
• Produits: Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Description de la lignée cellulaire

Mero-25 was derived from autopsy tumour material from a male patient aged >60 with a history of asbestos exposure

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,12
D5S818: 11,12
D7S820: 9
THO1: 8
TPOX: 9
vWA: 16,17

Milieu de culture

Hams F10 + 15% FCS + 2mM Glutamine

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 48 hours

Autres remarques

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