LCL721 human cell line

IHW Number: 9249, 09071301

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human blood
• Ethnicité: Not specified
• Sexe: female
• Mode de croissance: suspension
• Caryotype: Not specified
• Morphologie: Lymphoblastoid
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

Consanguineous: No

Homozygous: No

Description de la lignée cellulaire

An Epstein-Barr virus (EBV) transformed lymphoblastoid cell line with Human Leukocyte Antigen (HLA)-typing data available.

Milieu de culture

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442). To help establish a culture the concentration of FBS can be increased to 20%.

Procédure de repiquage

When resuscitating from frozen, cells should be seeded at 0.5 x 10⁶ cells/ml in the culture medium. The culture should establish log phase growth after 3-5 days. When growing well dilute every 1-3 days to 3 x 10⁵ cells/ml. Maintain cultures between 3-9 x 10⁵ cells/ml for maximum growth; 5% CO₂; 37°C. Cells form floating aggregates of varying size.

Autres remarques

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.