Vero (AC-free)

8011101, African green monkey kidney, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: Vero (AC-free), from African green monkey kidney, 08011101, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• Source biologique: African green monkey kidney
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Récepteurs: Not specified

Description

Description générale

Vero cells are anchorage-dependent cells derived from the kidney of African green monkey cells. They have applications in molecular and cell biology research. Vero cells are a useful host for the cultivation of Rabies virus, Reovirus and Japanese encephalitis virus.

Origine de la lignée cellulaire

African green monkey kidney cells, serum-free

Description de la lignée cellulaire

Vero cell line (Sigma catalog no. 84113001) adapted to grow in animal component free medium (VP-SFM; Gibco catalog no. 11681-020).

Milieu de culture

VP-SFM (Gibco cat no. 11681-020) + 4 mM L-Glutamine (G7513). When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO (D2650).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Autres remarques

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