The formulation of this medium is proprietary, and therefore, information about glucose, cholesterol, and lipid concentrations cannot be provided.
C-28077
Cancer Cell Line Medium XF
250 ml
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.81
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Forme
liquid
Conditionnement
pkg of 250 ml
Technique(s)
cell culture | mammalian: suitable
Conditions d'expédition
wet ice
Description générale
Cancer Cell Line Medium XF
Application
Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability. The medium consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium.
Qualité
All lots of the PromoCell Cancer Cell Line Medium XF are subjected to comprehensive quality control tests using a human cancer cell line. Each lot of PromoCell Cancer Cell Line Medium XF is tested for the ability to support proliferation and serial passage of MCF7 breast carcinoma cells on fibronectincoated dishes. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Stockage et stabilité
Store the Basal Medium at 2 to 8°C in the dark, store the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 2 to 8°C. For use, prewarm only an aliquot of the complete medium and keep the remaining medium refrigerated at 2 to 8°C.
Procédure de repiquage
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Code de la classe de stockage
10 - Combustible liquids
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Certificats d'analyse (COA)
Lot/Batch Number
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Zhikai Wang et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(4) (2022-01-21)
Cancer immunotherapy frequently fails because most carcinomas have few T cells, suggesting that cancers can suppress T cell infiltration. Here, we show that cancer cells of human pancreatic ductal adenocarcinoma (PDA), colorectal cancer, and breast cancer are coated with transglutaminase-2
Contenu apparenté
Defined culture conditions. Consistent growth of human cancer cell lines in PromoCell’s serum-free/xeno-free Cancer Cell Line Media-XF. Explore over 350 PromoCell products on Sigma.com
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Would I be able to find out the glucose, cholesterol and lipid concentrations in this media? I am doing an experiment where these need to be carefully titrated, and I need to know the starting amounts. Thank you.
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