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Key Documents

C0897

Sigma-Aldrich

Citrate Lyase from Klebsiella pneumoniae

lyophilized powder, ≥0.20 unit/mg solid

Synonyme(s) :

Citrate oxaloacetate lyase

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About This Item

Numéro CAS:
Numéro de classification (Commission des enzymes):
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

lyophilized powder

Activité spécifique

≥0.20 unit/mg solid

Poids mol.

>5 kDa

Température de stockage

2-8°C

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Application

Citrate Lyase from Klebsiella pneumoniae has been used in the digestion of low molecular weight human milk fraction (>5 kDa referred to as 5kF).

Actions biochimiques/physiologiques

Citrate lyase is found in several microorganisms and catalyzes the first step of citrate degradation, forming acetate and oxaloacetate. The enzyme contains 3 polypeptide subunits, α-subunit (a transferase), β-subunit (acyl lyase) and γ-subunit (acyl-carrier protein).

Définition de l'unité

One unit will convert 1.0 μmole of citrate to oxalacetate per min at pH 7.6 at 25 °C.

Forme physique

Lyophilized powder containing bovine serum albumin, sucrose, and MgSO4

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Citric acid mediates the iron absorption from low molecular weight human milk fractions
Palika R, et al.
Journal of Agricultural and Food Chemistry, 61(46), 11151-11157 (2013)
P Dimroth et al.
Proceedings of the National Academy of Sciences of the United States of America, 72(9), 3458-3462 (1975-09-01)
Citrate lyase [EC 4.1.3.6; citrate oxaloacetate-lyase (pro-3S-CH2-COO--leads to acetate)] from Klebsiella aerogenes has been dissociated with urea; the three different subunits, alpha-chain (molecular weight congruent to 54,000), beta-chain (molecular weight congruent to 32,000), and gamma-chain (acyl carrier protein; molecular weight
M Isabel Burguete et al.
Dalton transactions (Cambridge, England : 2003), (36)(36), 4027-4033 (2007-09-11)
A turn-on fluorescent indicator for citric acid (citrate) has been developed, displaying high emission enhancement (+1500%) and low interference by other carboxylates. The sensor is based on the non-emissive copper(II) complex of a fluorescent amino amide, which, upon addition of
M Suzuki et al.
Archives of microbiology, 175(1), 75-78 (2001-03-29)
Hydrogenobacter thermophilus strain TK-6 was observed to grow anaerobically on nitrate as an electron acceptor when molecular hydrogen was used as an energy source. Nitrite was detected as the product of a respiratory reaction. 15NO, 15N2O, and 15N2 were detected
Julia Gaye-Siessegger et al.
Isotopes in environmental and health studies, 40(3), 181-190 (2004-09-17)
The use of stable isotope techniques for the reconstruction of diets has increased over the last decade. However, isotopic ratios in an animal are not only affected by the composition of the feed, but also by the amount of feed

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