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B7554

Sigma-Aldrich

Anticorps anti-c-Myc

clone 9E10, purified from hybridoma cell culture

Synonyme(s) :

Anti-c-Myc, Monoclonal Anti-c-Myc−Biotin antibody produced in mouse

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.56

Source biologique

mouse

Conjugué

biotin conjugate

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

9E10, monoclonal

Forme

buffered aqueous solution

Espèces réactives

human

Concentration

0.8 mg/mL

Technique(s)

western blot (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells or E. coli cells expressing c-Myc tagged fusion protein

Numéro d'accès UniProt

P01106

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MYC(4609)

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Description générale

The Monoclonal Anti-c-Myc mouse antibody is isolated from ascites and conjugated to biotin. The antibody recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C terminal to the fusion protein.

Spécificité

Staining by immunoblotting is specifically inhibited by the c-Myc peptide (Product Code M2435).

Immunogène

peptide synthétique de la protéine p62c-Myc humaine.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
The antibody is suitable for immunoblotting.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Illari Salvatori et al.
Journal of neurochemistry, 146(5), 585-597 (2018-05-21)
TAR DNA-binding protein 43 (TDP-43) is an RNA-binding protein and a major component of protein aggregates found in amyotrophic lateral sclerosis and several other neurodegenerative diseases. TDP-43 exists as a full-length protein and as two shorter forms of 25 and
Yan Wang et al.
PloS one, 7(12), e52404-e52404 (2012-12-29)
The neural cell adhesion molecule L1 plays important roles in neuronal migration and survival, neuritogenesis and synaptogenesis. L1 has also been found in tumors of different origins, with levels of L1 expression correlating positively with the metastatic potential of tumors.
Steven R Bates et al.
Applied physics letters, 95(7), 73705-73705 (2009-09-25)
We used mechanical trapping of molecular interactions to demonstrate a highly parallel approach to measure the kinetics of biomolecular interactions. This approach consumes 25 fmol of material per measurement and permits 320 measurements in a single experiment. We measured association
Ilja Demuth et al.
Oncogene, 23(53), 8611-8618 (2004-10-07)
DNA interstrand crosslinks (ICLs) are critical lesions for the mammalian cell since they affect both DNA strands and block transcription and replication. The repair of ICLs in the mammalian cell involves components of different repair pathways such as nucleotide-excision repair
Romain Rouet et al.
Nature protocols, 7(2), 364-373 (2012-02-04)
Here we describe protocols for the expression of human antibody fragments in Escherichia coli. Antigen-specific clones are identified by soluble fragment ELISA and concentrated by periplasmic preparation. They are then further purified by affinity chromatography. This article provides an overview
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