B7185

Anti-Breast Cancer Resistance Protein antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-BCRP
• Numéro MDL: MFCD03791618
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~70 kDa
• Espèces réactives: mouse, human
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using heat-retrieved tissue sections from human term placenta by indirect immunoperoxidase staining of syncytiotrophoblasts.; western blot: 2.5-5 μg/mL using whole extract of human term placenta or mouse kidney and a chemiluminescent detection reagent
• Numéro d'accès UniProt: Q9UNQ0
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ABCG2(9429) ; mouse ... Abcg2(26357)

Description

Description générale

Anti-Breast Cancer Resistance Protein is developed in rabbit using as immunogen a synthetic peptide of human breast cancer resistance protein (BCRP). BCRP, also designated ABCG2, ABCP, placenta specific ATP-binding transporter and mitoxantrone-resistance protein (MXR), is a member of the ABCG subfamily of the large ATP binding cassette (ABC) transporter family of transmembrane proteins. BCRP is a half-transporter, with a single ATP binding cassette and transmembrane region. BCRP is normally expressed in human placental syncytiotrophoblasts, mammary ducts and lobules, endothelial cells and stem cells of different tissues.

Immunogène

synthetic peptide corresponding to amino acid residues 150-167 of human breast cancer resistance protein with C-terminal added cysteine, conjugated to KLH.

Application

Anti-Breast Cancer Resistance Protein antibody produced in rabbit has been used in:

• western blotting
• immunocytochemistry
• immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Actions biochimiques/physiologiques

Up regulation of breast cancer resistance protein (BCRP) expression may be found in vivo and in vitro in both drugs treated and untreated tumors of different types. Breast cancer resistance protein transports various xenobiotics to the extracellular space through a process energized by ATP hydrolysis. In addition, to its role in protecting cells and tissues against xenobiotics especially in the intestine, liver, placenta and the blood-brain barrier, BCRP appears to be involved in hypoxic defense mechanisms and the protection and regulation of stem cells.

Forme physique

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable