A7107

Monoclonal Anti-AP2 antibody produced in mouse

~1.0 mg/mL, clone A6/2/2, purified immunoglobulin, buffered aqueous solution

• Synonyme(s) : Anti-AP2TF, Anti-TFAP2
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: A6/2/2, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~50 kDa
• Espèces réactives: human
• Concentration: ~1.0 mg/mL
• Technique(s): immunohistochemistry: suitable; western blot: 2-4 μg/mL using G361 total cell extract
• Isotype: IgG1
• Numéro d'accès UniProt: P05549
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TFAP2A(7020)

Description

Description générale

Adaptor protein-2 (AP2) is a key constituent of clathrin-coated vesicles that mediates the endocytosis of cell membrane components such as G protein-coupled receptors (GPCRs). AP2 is a heterotetramer that consists of α, β, μ and σ subunits which bind to tyrosine- and dileucine-based motifs on membrane-associated cargo proteins.

Monoclonal Anti-AP2 (mouse IgG1 isotype) is derived from the hybridoma A6/2/2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to amino acids of human AP2α. In humans and mice, the adaptor protein-2 (AP2) family comprises five different transcription factors namely AP2α, AP2β, AP2 γ, AP2δ, and AP2ε. These proteins contain N-terminal transactivation domain and C-terminal helix-span-helix motif, which together with a central basic region facilitates dimerization and DNA binding.

Immunogène

peptide corresponding to amino acid 415-433 of human AP2α.

Application

Monoclonal Anti-AP2 antibody produced in mouse has been used in:

• immunofluorescence staining
• immunoblotting
• immunohistochemistry

Actions biochimiques/physiologiques

Adaptor protein-2 (AP2) expression is associated with the embryonic development. AP2β was found to be a tumor specific human telomerase reverse transcriptase (hTERT) promoter activator, suggesting it may be a biomarker for cancer diagnosis or as a cancer therapeutic target for inhibiting hTERT activity and tumor cell growth. In humans, mutations or loss of these genes result in increased tumor growth and metastasis. Specifically, AP2α loss causes down regulation of E-cadherin and matrix metallopeptidase 9 (MMP-9) activity, which in turn enhance tumorigenicity of colon cancer cells. This effect may also be the result of AP2α regulation by p53.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable