A7030
Synonyme(s) :
Albumine bovine, Albumine, sérum bovin, BSA, ASB
| À vous/Référence | Disponibilité | Prix |
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bovine
≥98%
lyophilized powder
heat shock fractionation
USA origin
≥95% protein
≤0.02%
ELISA: suitable, blood typing: suitable, ligand binding assay: suitable
essentially globulin free
≤5%
7
water: soluble (40 mg/ml)
protease ≤0.005 U/mg
2-8°C
bovine ... ALB(280717)
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Cet article | |||
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| description heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98% | description heat shock fraction, protease free, essentially globulin free, pH 7, ≥98% | description heat shock fraction, protease free, low endotoxin, suitable for cell culture, pH 7, ≥98% | description heat shock fraction, lyophilized powder, essentially fatty acid free, ≥98% (agarose gel electrophoresis) |
| assay ≥98% | assay ≥98% | assay ≥98% | assay ≥98% (agarose gel electrophoresis) |
| technique(s) ELISA: suitable, ligand binding assay: suitable, blood typing: suitable | technique(s) western blot: suitable, ELISA: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
Explore all of our products under Albumine de sérum bovin
11 - Combustible Solids
Not applicable
Not applicable
Eyeshields, Gloves
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This Bovine Serum Albumin is not tested for endotoxins. Please see the following options of BSA products which are tested for endotoxins:
https://www.sigmaaldrich.com/product/sigma/a8806
https://www.sigmaaldrich.com/product/sigma/a1933
https://www.sigmaaldrich.com/product/sigma/a2934
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The stability of this material in solution, when stored at -70 °C, has not been evaluated. However, Albumin containing solutions are generally stable when stored at -20 to -80 °C in single use aliquots for at least 1 year. Also note that a number of sterile prepared BSA solutions currently available may be stored at 2–8 °C with assigned retest dates that range from 2 – 4 years from the date of Quality Release. Albumin is quite stable in solution and freezer storage may not be necessary.
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The minimum allowable free fatty acid content is ≤ 0.02% (w/w), as determined by enzymatic assay. A review of recent lots indicates typical values of 0.00% free fatty acids. This information is lot-specific and reported in the Certificate of Analysis. Please see the link below to access a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/a7030#product-documentation
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This product is not routinely tested for Biotin. However, this material is dialyzed with a 10 kDa cutoff membrane. This dialysis step removes small molecules such as biotin, which has a molecular weight of less than 500 Da. Therefore, this product is considered low in biotin, helping to prevent interference with streptavidin–biotin binding.
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This product is shipped at ambient temperature and is stable at ambient temperature for up to two weeks. For long term storage refrigerated temperature is recommended.
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If stored at 2-8 °C, a filter sterilized 20% BSA solution in water or aqueous buffer is generally stable for a minimum of 2.5 - 3 years.
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The magnesium content of this material is not routinely tested, but historical data indicates that three lots contained between 10-20 parts per million. Water is used as the pre-lyophilization solvent.
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Is product number A7030, Bovine Serum Albumin (BSA) glycosylated?
Unfortunately, there is currently no information available regarding the glycosylation status of BSA product A7030.
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The specific method used to test this material for protease activity is considered proprietary. However, it is based on the enzymatic cleavage of FITC-Casein. See the link below to review the FITC-Casein Enzymatic Assay to demonstrate the principal of this method.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/353/850/c3777enz.pdf
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This product is not assigned an expiration date. It is assigned a recommended retest date of 5 years after the quality release date. The recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
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