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A1420

Sigma-Aldrich

Ames′ Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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About This Item

Code UNSPSC :
41161501
Nomenclature NACRES :
NA.75

Niveau de qualité

500

Forme

powder

Technique(s)

cell culture | mammalian: suitable

Composants

glucose: 1.081 g/L (Dextro)
NaHCO3: no
L-glutamine: 0.073 g/L

Conditions d'expédition

ambient

Température de stockage

2-8°C

Catégories apparentées

Description générale

Ames′ Medium has been formulated to support retinal tissue in relatively short-term culture. It is suitable for maintaining central nervous system tissue in vitro.

Application

Ames′ Medium has been used to maintain rat/mice/macaque monkey eye for the isolation of retina.

Quantité

Formulated to contain 8.8 grams of powder per liter of medium.

Reconstitution

Supplement with 1.9 g/L sodium bicarbonate.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Qiuju Jiang et al.
IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society, 26(5), 969-976 (2018-05-13)
Significant progress has been made recently in treating neurological blindness using implantable visual prostheses. However, implantable medical devices are highly invasive and subject to many safety, efficacy, and cost issues. The discovery that ultrasound (US) may be useful as a
Antoine Chaffiol et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 25(11), 2546-2560 (2017-08-16)
The majority of inherited retinal degenerations converge on the phenotype of photoreceptor cell death. Second- and third-order neurons are spared in these diseases, making it possible to restore retinal light responses using optogenetics. Viral expression of channelrhodopsin in the third-order
Frans Vinberg et al.
Vision research, 101, 108-117 (2014-06-25)
The Function of the retina and effects of drugs on it can be assessed by recording transretinal voltage across isolated retina that is perfused with physiological medium. However, building ex vivo ERG apparatus requires substantial amount of time, resources and
Kasey Rose et al.
Molecular neurodegeneration, 12(1), 28-28 (2017-04-13)
Light exposure triggers movement of certain signaling proteins within the cellular compartments of the highly polarized rod photoreceptor cell. This redistribution of proteins between the inner and outer segment compartments affects the performance and physiology of the rod cell. In
Shijun Weng et al.
Journal of biological rhythms, 24(5), 391-402 (2009-09-17)
Intrinsically photosensitive retinal ganglion cells (ipRGCs) project to the suprachiasmatic nucleus (SCN) and are essential for normal photic entrainment of global circadian rhythms in physiology and behavior. The effect of light on the central clock is dependent on circadian phase
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Protocoles

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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