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A1054

Sigma-Aldrich

Anti-Digoxin–Alkaline Phosphatase antibody, Mouse monoclonal

clone DI-22, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-Digoxin, Monoclonal Anti-Digoxin–Alkaline Phosphatase antibody produced in mouse

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Produit recombinant

expressed in mouse cell line

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

DI-22, monoclonal

Forme

buffered aqueous solution

Technique(s)

direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate
direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate
dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)

Isotype

IgG1

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Digoxin is a cardiac glycoside that is used to treat chronic heart failure.Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody can be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies and peptides. The antibody is specific for digoxin and digoxin-labeled compounds. The product shows strong cross-reactivity with digoxigenin.

Spécificité

Antibody is specific for digoxin and shows a high affinity for digoxigenin.

Immunogène

Digoxin-KLH.

Application

Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:10000-1:6000) and dot blot (at 1:20,000 dilutions using digoxigenin-labeled biomolecules). It has also been used for enzyme immunoassay (EIA).
Nested PCR products were hybridized with 5′-end digoxigenin-labeled oligonucleotide probe. ELISA assays were performed to detect the probe by adding monoclonal anti-digoxin antibody alkaline phosphatase (DI-22) diluted 1:1000 in 1 M phosphate-buffered saline, 1% bovine serum albumin, and 0.1% Tween 20 (Ph 7.4). Detection was performed using Sigma Fass-Pnpp substrate tablets.

Forme physique

Solution in 0.05 M Tris buffer, pH 8.0 containing 1% BSA, 50% glycerol, 1 mM MgCl2 and 15 mM sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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J N Mbatha et al.
BMC research notes, 10(1), 702-702 (2017-12-07)
Cervical cancer is a major problem in women and it is important to find a suitable and acceptable screening method, especially among young in low-resource areas for future human papillomavirus (HPV) vaccine follow-up investigations. The study sought to test the
Joyce N Mbatha et al.
Infectious diseases (London, England), 49(8), 601-608 (2017-04-14)
High-risk human papillomavirus (hr-HPV) infections and low-grade squamous intraepithelial lesions occur frequently in young women. The available vaccines cover up to seven hr-HPV genotypes (HPV16, HPV18, HPV31, HPV33, HPV45, HPV52 and HPV58) and two low-risk HPV types (HPV6 and HPV11).
The New England journal of medicine, 336(8), 525-533 (1997-02-20)
The role of cardiac glycosides in treating patients with chronic heart failure and normal sinus rhythm remains controversial. We studied the effect of digoxin on mortality and hospitalization in a randomized, double-blind clinical trial. In the main trial, patients with
Liang Wang et al.
Theranostics, 11(2), 925-940 (2021-01-05)
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