89050907

LLC-RK1 cell line

rabbit kidney, Epithelial-like

• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.81

Propriétés

• product name: LLC-RK1 cell line, from rabbit kidney
• Source biologique: rabbit kidney
• Forme: liquid
• Mode de croissance: Adherent
• Caryotype: Modal no. 45 (2n=46)
• Morphologie: Epithelial-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description de la lignée cellulaire

Derived from kidneys taken from New Zealand white rabbits. The cells are susceptible to a range of viruses including rubella, vaccinia and herpes. Rubella replicates to a high titre.

Milieu de culture

Medium 199 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 5x10,000 to 3x100,000 cells/cm²using 0.25% trypsin; 5% CO₂; 37°C. NB: this line is extremely sensitive to trypsin.

Autres remarques

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Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable