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83200

Pyronin Y

suitable for microscopy

Synonyme(s) :

Pyronin G, Pyronin J

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A propos de cet article

Formule empirique (notation de Hill) :
C17H19ClN2O
Numéro CAS:
Poids moléculaire :
302.80
EC Number:
202-147-8
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3795789
Colour Index Number:
45005
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mp

250-260 °C (lit.)

solubility

water: 0.01 g/10 mL, red

εmax

≥1650 at 543-553 nm in 50% ethanol, ≥650 at 249-259 nm in 50% ethanol

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

Biochem/physiol Actions

Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.

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Cet article
213519P917222680
suitability

suitable for microscopy

suitability

-

suitability

suitable for nucleic acid staining

suitability

suitable for microscopy

solubility

water: 0.01 g/10 mL, red

solubility

-

solubility

water: 10 mg/mL, dark red

solubility

H2O: 0.1 g/10 mL

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

mp

250-260 °C (lit.)

mp

250-260 °C (lit.)

mp

250-260 °C (lit.)

mp

-

εmax

≥1650 at 543-553 nm in 50% ethanol

εmax

-

εmax

-

εmax

≥350 at 283-293 nm in water, ≥450 at 407-417 nm in water


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Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Consulter la Bibliothèque de documents



Rost FWD
Fluorescence Microscopy (1995)
Karsten Kuritz et al.
Scientific reports, 10(1), 3619-3619 (2020-02-29)
Modern cytometry methods allow collecting complex, multi-dimensional data sets from heterogeneous cell populations at single-cell resolution. While methods exist to describe the progression and order of cellular processes from snapshots of such populations, these descriptions are limited to arbitrary pseudotime
Myron Nevins et al.
Journal of periodontology, 83(7), 878-884 (2012-01-10)
This study tests the effectiveness of hydroxyapatite and collagen bone blocks of equine origin (eHAC), infused with recombinant human platelet-derived growth factor-BB (rhPDGF-BB), to augment localized posterior mandibular defects in non-human primates (Papio hamadryas). Bilateral critical-sized defects simulating severe atrophy



Numéro d'article de commerce international

RéférenceGTIN
83200-10G04061833005446
83200-5G04061833005453

Questions

1–5 of 5 Questions  
  1. What is the emission wavelength of Pyronin Y, Product 83200?

    1 answer
    1. The emission is at 570 nm when dissolved in water.

      Helpful?

  2. Has Pyronin Y, Product 83200, been use-tested for the demonstration of RNA?

    1 answer
    1. No. There are, however, two other Pyronin products from Sigma-Aldrich which should be suitable.  Product No. P9172 has been used to stain RNA in polyacrylamide gels.  Product No. 213519 was submitted to the Biological Stain Commission for certification.  Testing was performed by the Biological Stain Commission to ensure the Pyronin Y stained RNA in tissue sections.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. What is the solubility of Pyronin Y, Product 83200, in water and ethanol?

    1 answer
    1. Solubility is not tested at Sigma Aldrich, but the literature reports solubility up to 9% (i.e., 90 mg/mL) in water and 0.6% (6 mg/mL) in ethanol.

      Helpful?

  5. Is there a prepared solution of Methyl Green and Pyronin Y for the demonstration of plasma cells and RNA in tissue sections?

    1 answer
    1. Yes, product HT70116 is available in pre-made form.  The product is approved for in vitro diagnostic (IVD) use.

      Helpful?

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