60700

Nuclear Fast Red

for microscopy (Bot., Hist.)

• Synonyme(s) : 4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt
• Formule empirique (notation de Hill): C₁₄H₈NNaO₇S
• Numéro CAS: 6409-77-4
• Poids moléculaire : 357.27
• Numéro C.I. (Colour Index): 60760
• Numéro CE : 229-088-0
• Numéro MDL: MFCD00078493
• Code UNSPSC : 12171500
• ID de substance PubChem : 57651779
• Nomenclature NACRES : NA.47

Propriétés

• Qualité: for microscopy (Bot., Hist.)
• Forme: powder
• Solubilité: methanol: water (1:1): 0.01 g/10 mL, red
• εmax: ≥275 at 550-560 nm in DMSO; ≥300 at 516-526 nm in DMSO
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Na+].Nc1c(O)c(c(O)c2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O
• InChI: 1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1
• Clé InChI: IFSXZLJQEKGQAF-UHFFFAOYSA-M

Description

Description générale

Nuclear Fast Red (C.l. 60760) is a substituted hydroxy anthraquinone chromophore that can chelate metal ions. It is also known as kernechtrot and 4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt. It is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is a dry staining dye that makes target structures (by fixing, wherenecessary embedding, staining with the above nuclear fast red solution, counterstaining, mounting) in human-histological specimen materials, for examplehistological sections of e. g. the kidney, evaluable for diagnostic purposes.

Application

Nuclear fast red has been used for the nuclear staining of PB (Prussian blue) positive cells in the rostral sub-epidermis and upper beak coronal region isolated from pigeons.Recent applications include:

• staining sperm and spermatids in semen
• identifying areas for PCR analysis in paraffin-embedded tissues
• nuclear counterstaining following immunostaining and silver staining

Used as a biological stain

Actions biochimiques/physiologiques

Nuclear fast red dye has anthraquinonic nature and is used either alone or in combination with other dyes. It is mainly used for staining of nuclei.

Principe

Nuclear fast red can be used for simple nuclear staining. It is an acidic dyebelonging to the group of anthraquinone dyes. Aluminum sulfate, functioning as a mordant, is added to form a color lake that enables red nuclear staining. Nuclear fast red-aluminum sulfate is used for counterstaining in histochemicalreactions, e. g. in Berlin blue staining for detection of iron or in silver staining. Nuclear fast red for microscopy Certistain^® can also be used fornuclear staining with alcian blue.

Informations légales

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves