D9564

DAPI, dilactate

≥98% purity (HPLC), powder

• Synonyme(s) : 4′,6-Diamidino-2-phenyindole, dilactate
• Formule empirique (notation de Hill) : C₁₆H₁₅N₅ · 2C₃H₆O₃
• Poids moléculaire : 457.48
• MDL number: MFCD00467366
• UNSPSC Code: 12171500
• PubChem Substance ID: 329798905
• NACRES: NA.47

Propriétés

• Nom du produit: DAPI, dilactate, ≥98% (HPLC)
• Quality Segment: 300
• assay: ≥98% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: yellow
• solubility: H₂O: 20 mg/mL
• ε (extinction coefficient): 28.00-32.50 at 345-351 nm in methanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
• InChI: InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)
• InChI key: LCAUPANIXBMNIA-UHFFFAOYSA-N

Description

General description

DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.

Application

DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.

Biochem/physiol Actions

A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)