69198

Fraser Broth, Base

suitable for microbiology, NutriSelect^® Plus

• Synonyme(s) : Listeria Selective Enrichement Broth, Base (acc. Fraser)
• Code UNSPSC : 41171606
• Nomenclature NACRES : NA.85

Propriétés

• Stérilité: non-sterile
• Niveau de qualité: 200
• Forme: powder
• Durée de conservation: limited shelf life, expiry date on the label
• Composition: casein enzymic hydrolysate, 5.0 g/L ; disodium phosphate dihydrate, 12.0 g/L ; esculin, 1.0 g/L ; lithium chloride, 3.0 g/L ; meat extract, 5.0 g/L ; peptic digest of animal tissue, 5.0 g/L ; potassium dihydrogen phosphate, 1.35 g/L ; sodium chloride, 20.0 g/L ; yeast extract, 5.0 g/L
• Fabricant/nom de marque: NutriSelect^® Plus
• Technique(s): microbiological culture: suitable
• pH final: 7.2±0.2 (25 °C)
• Application(s): agriculture; environmental; food and beverages; pathogen testing; sample preparation; microbiology
• Adéquation: selective and differential for Listeria spp.

Description

Application

Fraser Broth Base with added supplement is recommended by ISO Committee for primary and secondary enrichment medium, for the isolation and enumeration of Listeria monocytogenes from food and animal feeds

Notes préparatoires

Suspend 57.35 grams of dehydrated medium in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Fraser Selective Supplement (Cat. No. 18038) and 2 vials of Fraser Supplement (Cat. No. 90836) to 1000 ml medium for primary enrichment or 1 vial of each to 500 ml medium for secondary enrichment. Mix well and dispense as desired.
Warning : Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin wash with plenty of water immediately.

Note de bas de page

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)