MABT333

Anti-CD167a/DDR1 Antibody, clone 5D5

clone 5D5, from mouse

• Synonyme(s) : Epithelial discoidin domain-containing receptor 1, CD167 antigen-like family member A, CD167a, Cell adhesion kinase, Discoidin receptor tyrosine kinase, Epithelial discoidin domain receptor 1, HGK2, Mammary carcinoma kinase 10, MCK-10, Protein-tyrosine k
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 5D5, monoclonal
• Espèces réactives: human
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001945.3
• Numéro d'accès UniProt: Q08345
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DDR1(780)

Description

Description générale

Epithelial discoidin domain-containing receptor 1 (EC 2.7.10.1; UniProt Q08345; also known as CD167 antigen-like family member A, CD167a, Cell adhesion kinase, Discoidin receptor tyrosine kinase, Epithelial discoidin domain receptor 1, HGK2, Mammary carcinoma kinase 10, MCK-10, Protein-tyrosine kinase 3A, Protein-tyrosine kinase RTK-6, TRK E, Tyrosine kinase DDR, Tyrosine-protein kinase CAK) is encoded by the DDR1 (also known as CAK, EDDR1, MCK10, NEP, NTRK4, PTK3A, RTK6, TRKE) gene (Gene ID 780) in human. Discoidin domain-containing receptors (DDRs) belong to the superfamily of transmembrane receptor tyrosine kinases (RTKs). DDRs are distinguished from other RTKs by their extracellular discoidin motif and their utilization of collagens as internal ligands. While DDR1 binds essentially all types of collagens, DDR2 shows a preference for type I, II and III fibrillar collagens, and nonfibrillar type X collagen. DDR1 is widely expressed in epithelial cells in lung, kidney, colon, and brain, whereas DDR2 is primarily expressed in mesenchymal cells including fibroblasts, myofibroblasts, smooth muscle cells, and chondrocytes in kidney, skin, lung, heart, and connective tissues. DDRs are important mediators of fundamental cellular processes, such as proliferation, survival, differentiation, adhesion and matrix remodeling, their dysregulations are reported to cause a number of human diseases, including fibrotic disorders, atherosclerosis and cancer. DDR1 is initially produced with a signal peptide sequence (a.a. 1-20), the removal of which yields the mature protein with an extracellular region (a.a. 21-417) that contains the Discoidin domain (F5/8 type C domain or C2-like domain) at the N-terminal half (a.a. 31-185) and a collagen-binding site within the membrane-distal DS domain (a.a. 192-367), followed by a transmembrane segment (a.a. 418-438) and a cytoplasmic region (a.a. 439-913) that harbors a WW domain-interacting PPxY motif (a.a. 481-484) and the kinase domain (a.a. 610-905). Alternative splicings produce five isoforms, isoforms 2 (CAK II, DDR1a, Short) and 5 are expressed with a truncated cytoplasmic domain, while isoform 3 (DDR1d) is expressed in a 243-a.a. soluble form and isoform 4 is expressed with a 6-a.a. insertion (SFSLFS) between Ala665 and Arg666 of the canonical isoform 1 (CAK I, DDR1b) sequence.

Spécificité

Clone 5D5 bound recombinant human DDR1b extracellular domain without affinity toward DDR2 extracellular domain or DDR1b membrane-distal DS domain containing the collagen-binding site (Carafoli, F., et al. (2012). Structure. 20(4):688-697). Clone 5D5 does not recognize denatured DDR1b and therefore is not suitable for Western blotting application.

Immunogène

Recombinant human DDR1b extracellular domain.

Épitope : domaine extracellulaire

Application

Analyse par immunocytochimie : µ

A representative lot bound immobilized recombinant human DDR1b extracellular domain (Kd ~2 nM) without affinity toward DDR2 extracellular domain or DDR1b membrane-distal DS domain containing the collagen-binding site (Carafoli, F., et al. (2012). Structure.

A representative lot detected the exogenously expressed DDR1b wild-type and mutant constructs on the surface of transfected HEK293 cells (Carafoli, F., et al. (2012). Structure.

A representative lot inhibited collagen-induced autophosphorylation of DDR1b exogenously expressed on the surface of transfected HEK293 cells (Carafoli, F., et al. (2012). Structure. 20(4):688-697).

Domaine de recherche
Structure cellulaire

This mouse monoclonal Anti-CD167a/DDR1, clone 5D5 Antibody, Cat. No. MABT333 is validated for use in ELISA, Flow Cytometry, Immunocytochemistry, and Neutralization applications.

Qualité

µ

Description de la cible

99.18/101.1 kDa (mature/pro-isoform 1, CAK I, DDR1b), 95.22/97.17 kDa (mature/pro-isoform 2, CAK II, DDR1a, Short), 25.18/27.13 kDa (mature/pro-isoform 3, DDR1d), 99.84/101.8 kDa (mature/pro-isoform 4), 97.11/99.06 kDa (mature/pro-isoform 5) calculated.

Forme physique

Format : Produit purifié

Produit purifié sur protéine G.

Purified mouse IgG1 in PBS without preservatives.

Stockage et stabilité

Stable à -20 °C pendant 1 an à compter de la date de réception.

Recommandations relatives à la manipulation du produit : Dès réception, et avant le retrait du bouchon, centrifuger le flacon et mélanger délicatement la solution. Répartir en aliquotes dans des microtubes à centrifuger et conserver ces derniers à -20 °C. Éviter les congélations/décongélations répétées, qui peuvent détériorer les IgG et nuire aux performances du produit.

Autres remarques

Concentration : Voir la fiche technique du lot concerné.

Clause de non-responsabilité

Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable