MABF985

Anti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5

clone 6D1.5/A5, from mouse

• Synonyme(s) : Non-secretory ribonuclease, Eosinophil-derived neurotoxin, Ribonuclease 2, Ribonuclease US, RNase 2, RNase UpI-2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 6D1.5/A5, monoclonal
• Application: ELISA, WB, multiplexing
• Citations: -

Propriétés

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6D1.5/A5, monoclonal
• species reactivity: human
• technique(s): ELISA: suitable, multiplexing: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NP_002925
• UniProt accession no.: P10153
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RNASE2(6036)

Description

General description

Non-secretory ribonuclease (EC 3.1.27.5; UniProt P10153; also known as Eosinophil-derived neurotoxin, Ribonuclease 2, Ribonuclease US, RNase 2, RNase UpI-2) is encoded by the RNASE2 (also known as EDN, RAF3, RNS2) gene (Gene ID 6036) in human. The major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil derived neurotoxin (EDN) and eosinophil peroxidase (EPO), are eosinophil effector molecules implicated in host defense, immunoregulatory responses, and in allergic and inflammatory reactions. “” While eosinophil count and eosinophil surface expression of the activation marker CD69 are significantly correlated with serum concentrations of MBP, EDN and EPO, studies show that they are not correlated with ECP level. On the other hand, eosinophilic disease patients with normal eosinophil count show significantly increased concentrations of MBP, ECP, EDN, and EPO compared to normal donors. The measurement of MBP, ECP, EDN, and EPO levels thus provides a better predictor than eosinophil count alone in disease diagnosis. EDN is produced with a signal peptide sequence (a.a. 1-27), the removal of which yields the mature (a.a. 28-161) protein.

Poids réel (observé) : env. 20 kDa. Target band appears larger than the calculated molecular weights of 18.35 kDa (pro-form) and 15.46 kDa (mature) due to glycosylation. Une ou plusieurs bandes non caractérisées peuvent apparaître avec certains lysats.

Immunogen

Purified human EDN.

Application

Analyse par western blotting : µµ
A representative lot was employed as the capture antibody for the detection of purified human EDN, as well as EDN in serum samples from individuals with eosinophilic disorders by sandwich ELISA (Makiya, M.A., et al. (2014). J. Immunol. Methods.
A representative lot was employed as the capture antibody in a bead-based multiplex assay for the detection of purified human EDN as well as EDN in serum samples from healthy donors and individuals with eosinophilic disorders. Prior reduction and alkylation of the serum samples (by DTT and iodoacetamide, respectively) resulted in higher EDN levels (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22),

Anti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5 is an antibody against Eosinophil-Derived Neurotoxin for use in Western Blotting, ELISA, Multiplexing.

Domaine de recherche
Inflammation et immunologie

Sous-domaine de recherche
Inflammation et mécanismes auto-immuns

Biochem/physiol Actions

Clone 6D1.5/A5 specifically reacted with EDN, but not other granule proteins by Western blotting analysis (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22).

Physical form

Format : Produit purifié

Produit purifié sur protéine G

Purified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable entre 2 et 8 °C pendant 1 an à compter de la date de réception.

Analysis Note

Produit évalué par western blotting sur du lysat de tissu de rate humaine.

Analyse par western blotting : µµ

Other Notes

Concentration : Voir la fiche technique du lot concerné.

Disclaimer

Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.

Informations sur la sécurité

• Que se passe-t-il ?: nwg
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash
• Classe de stockage: 12 - Non Combustible Liquids