MABE1893

Anti-RFC1 Antibody

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 6, monoclonal
• Poids mol.: calculated mol wt 128.26 kDa; observed mol wt ~140 kDa
• Espèces réactives: human, rat
• Conditionnement: antibody small pack of 100 μL
• Technique(s): western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès UniProt: P35251
• Conditions d'expédition: dry ice
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... RFC1(5981)

Description

Description générale

Replication factor C subunit 1 (UniProt: P35251; also known as Activator 1 140 kDa subunit, A1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1, DNA-binding protein PO-GA, Replication factor C 140 kDa subunit, RF-C 140 kDa subunit, RFC140, Replication factor C large subunit) is encoded by the RFC1 (also known as RFC140) gene (Gene ID: 5981) in human. Replication factor C (RFC) is a multi-subunit DNA replication factor that is composed of five subunits of 140, 40, 38, 37, and 36 kDa size. These five subunits exist in all eukaryotes and are highly conserved in both structure and function. RFC1 is the largest subunit in this complex. RFC serves as a DNA-dependent ATPase that binds in a structure-specific manner to the 3 end of a primer hybridized to a template DNA. This ATPase is activated in the presence of primer/template DNA and is further stimulated by PCNA addition. In eukaryotes, it acts as a clamp loader that can bind and open the homotrimeric PCNA clamp, and then load PCNA onto a template-primer junction in an ATP-dependent reaction. Coupled with ATP hydrolysis, RFC is ejected from the PCNA clamp for the next round of DNA synthesis and then the clamp recruits DNA polymerases for processive elongation of DNA chains. In addition to its role in DNA replication, RFC also plays a role in DNA polymerase switching, DNA repair, and check-point control in cell cycle progression. RFC1 displays wide tissue distribution but is undetectable in placental tissue. (Ref.: Chen, Y., et al. (2018). Front. Plant Sci. 9; 779; Bunz, F., et a. (1993). Proc. Natl. Acad. Sci. USA. 90 (23); 11014-11018; Tsurimoto, T., and Stillman, B. (1989). EMBO J. 8(12); 3883-3889).

Spécificité

Clone 6 is a mouse monoclonal antibody that detects Replication factor C subunit 1 (RFC1).

Immunogène

Purified Replication factor C subunit 1 (RFC1) from HEK293 cell nuclear extract.

Application

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysates.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Replication factor C subunit 1 (RFC1) in HepG2 cell lysates.

Tested Applications

Western Blotting Analysis: A representative lot detected RFC1 in Western Blotting applications (Farina, A., et. al. (2008). J Biol Chem. 283(30):20925-36).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-RFC1, clone 6, Cat. No. MABE1893, is a mouse monoclonal antibody that detects Replication factor C subunit 1 (RFC1) and is tested for use in Western Blotting.

Forme physique

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable