MABC519

Anti-Trop2, clone 2G10, Azide Free Antibody

clone 2G10, from mouse

• Synonyme(s) : Tumor-associated calcium signal transducer 2, Cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 2G10, monoclonal
• Espèces réactives: human
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; inhibition assay: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_002344
• Numéro d'accès UniProt: P09758
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TACSTD2(4070)

Description

Description générale

The protein known as Trop-2, or also known as cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1 or Pancreatic carcinoma marker protein GA733-1 and encoded by the gene TACSTD2/GA733-1/M1S1/TROP2, is a protein that may function as a growth factor receptor and is expressed primarily in the placenta, lung and epithelial tissues but also found in certain cancers. The protein was first identified as a carcinoma antigen by the monoclonal GA733. It is a plasma membrane bound protein that transduces an intracellular calcium signal after binding unknown ligands. Mutations in Trop2 are associated with a rare eye disease leading to blindness. Trop-2 has also recently been shown to be important in regulating fibroblast motility and lung cell proliferation via the regulation of the ERK kinase pathway as well as several critical components of the cell migration apparatus.

Immunogène

Recombinant protein corresponding to the extracellular domain of human Trop2.

Application

Immunocytochemistry Analysis: A representative lot detected Trop2 in MTE 4-14/T2 and MCF7 cells (Prof. Saverio Alberti, Oncoxx Biotec.).

Flow Cytometry Analysis: A representative lot detected Trop2 in transfected cells, Skov-3 cancer cells, and HT29 cancer cells (Prof. Saverio Alberti, Oncoxx Biotec.).

Inhibition Analysis: A representative lot inhibited tumor growth in DU145 prostate cancer cells, and in HT-29, HCT116 and Colo205 cells. (Note: When used together, Cat. No. MABC519, Anti-Trop2, clone 2G10, Azide Free and Cat. No. MABC518, Anti-Trop2, clone 2EF, Azide Free maximizes inhibition of tumor growth in vivo.) (Prof. Saverio Alberti, Oncoxx Biotec.).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Trop2 antibody, clone 2G10, Azide Free is validated for use in ICC, flow cytometry & inhibition for the detection of Trop2.

Qualité

Evaluated by Immunocytochemistry Analysis in MET cells transfected with a vector expressing Trop2 or with an empty vector.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Trop 2 in MET cells transfected with a vector expressing Trop2 and not in MET cells transfected with an empty vector.

Description de la cible

36 kDa calculated

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable