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Key Documents

MAB3069

Sigma-Aldrich

Anti-Connexin 32 Antibody, clone M12.13

clone M12.13, Chemicon®, from mouse

Synonyme(s) :

Cx32

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

M12.13, monoclonal

Espèces réactives

human, mouse, rat

Fabricant/nom de marque

Chemicon®

Technique(s)

immunohistochemistry: suitable
western blot: suitable

Isotype

IgG

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... GJB1(2705)

Spécificité

Monoclonal antibody MAB3069 was produced by the immunization of BALB/c mice with isolated rat junctional complexes, prepared by the method of Stevenson and Goodenough (1988). Epitope mapping, hydropathy plotting, and topological studies suggest that this antibody is very likely directed against an epitope located between residues 95-125 in the central cytoplasmic loop of rat Cx32.

Application

Anti-Connexin 32 Antibody, clone M12.13 detects level of Connexin 32 & has been published & validated for use in WB, IH.
Immunohistochemistry: acetone or unfixed tissues recommended. Epitope is internal, paraffin sections are untested.

Works with lightly formaldehyde-fixed tissue.

Western blot: Detects a 27 kDa Connexin 32 polypeptide in blots of rat hepatocyte gap junctions and an additional 47 kDa band corresponding to aggregates.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Forme physique

Format: Purified
Liquid in 0.02M PB, 0.25M NaCl, containing 0.1% sodium azide.

Stockage et stabilité

Maintain at 2-8°C in convenient undiluted aliquots for up to 6 months.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

J E Rash et al.
Neuroscience, 147(4), 938-956 (2007-07-03)
Locus coeruleus neurons are strongly coupled during early postnatal development, and it has been proposed that these neurons are linked by extraordinarily abundant gap junctions consisting of connexin32 (Cx32) and connexin26 (Cx26), and that those same connexins abundantly link neurons
W Matsuyama et al.
Journal of human genetics, 46(6), 307-313 (2001-06-08)
To clarify the pathomechanism in three patients with X-linked Charcot-Marie-Tooth disease (CMTX) and unique clinical features, we studied three connexin (Cx) 32 (GJB1) mutants with respect to cellular localization in cultured cells. Wild-type Cx32 and three Cx32 mutants (Va163Ile and
X Li et al.
Neuroscience, 126(3), 611-630 (2004-06-09)
Gap junctions between glial cells in mammalian CNS are known to contain several connexins (Cx), including Cx26, Cx30 and Cx43 at astrocyte-to-astrocyte junctions, and Cx29 and Cx32 on the oligodendrocyte side of astrocyte-to-oligodendrocyte junctions. Recent reports indicating that oligodendrocytes also
David Campard et al.
Gastroenterology, 134(3), 833-848 (2008-02-05)
Umbilical cord matrix stem cells (UCMSCs) are able to differentiate into mesodermal and ectodermal lineages. The present study investigates the differentiation potential of human UCMSCs into hepatic lineage. We isolated human UCMSCs and characterized them in vitro by measuring their
Lorena Rela et al.
Glia, 58(6), 665-678 (2009-12-10)
Olfactory ensheathing cells (OECs) have been repeatedly implicated in mediating plasticity, particularly in situ in the olfactory nerve in which they support the extension of olfactory sensory neuron (OSN) axons from the olfactory epithelium to the olfactory bulb (OB). OECs

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