CBL218-I
Anti-Keratin K3/K76 Antibody, clone AE5
clone AE5, from mouse
Synonyme(s) :
65 kDa cytokeratin, Cytokeratin-3, CK-3, Keratin-3, K3, Type-II keratin Kb3
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
AE5, monoclonal
Espèces réactives
rabbit, bovine
Technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... KRT3(3850)
Catégories apparentées
Description générale
Cytokeratin 3, also known as 65kDa Cytokeratin or Keratin K3 is a specific keratin found primarily in the corneal epithelium and minorly in other epithelium like that of the esophagus. The corneal epithelium tissue forms the outermost layer of the cornea, which is the clear front covering of the eye. Keratins are divided into two primary types based upon their isoelectric mobility and size, acidic, or type 1:40-55kDa, and basic to neutral or type 2, 56-70kDa. Cytokeratin 3 is a type II cytokeratin. Type II cytokeratins form pairs with other cytokeratins. Cytokeratin 3 is expressed in the corneal epithelium with Cytokeratin 12. Together Cytokeratin 3 and Cytokeratin 12 form molecules known as intermediate filaments. These filaments assemble into strong networks that provide strength and resilience to the corneal epithelium. ′
Immunogène
Whole cells corresponding to Rabbit Keratin, type II cytosleletal 3.
Application
Analyse par immunohistochimie :
A representative lot was used by an independent laboratory in Keratin 3 expressed epithelial cells on the surface of submerged and AL explants, indicating that they were derived from limbal progenitor cells via migration.
A representative lot was used by an independent laboratory in Rabbit epithelial Keratin lysate. (Schermer, A, et al. (1986) J. Cell Biol., 103:
A representative lot was used by an independent laboratory in rabbit corneal tissue lysate. (Cooper, D., et al. (1986) J. Biol. Chem., 261:
A representative lot was used by an independent laboratory in human limbal stem cells cultured in medium supplemented with human EGF. (Sharifi, A.M., et al. (2010) Biocell.
A representative lot was used by an independent laboratory in rabbit corneal and limbal tissues, limbal explant, and epithelial outgrowth on human AM.
A representative lot was used by an independent laboratory inRabbit corneal epithelial Keratin cells. (Schermer, A, et al. (1986) J. Cell Biol., 103: 49-62).
A representative lot was used by an independent laboratory in Keratin 3 expressed epithelial cells on the surface of submerged and AL explants, indicating that they were derived from limbal progenitor cells via migration.
A representative lot was used by an independent laboratory in Rabbit epithelial Keratin lysate. (Schermer, A, et al. (1986) J. Cell Biol., 103:
A representative lot was used by an independent laboratory in rabbit corneal tissue lysate. (Cooper, D., et al. (1986) J. Biol. Chem., 261:
A representative lot was used by an independent laboratory in human limbal stem cells cultured in medium supplemented with human EGF. (Sharifi, A.M., et al. (2010) Biocell.
A representative lot was used by an independent laboratory in rabbit corneal and limbal tissues, limbal explant, and epithelial outgrowth on human AM.
A representative lot was used by an independent laboratory inRabbit corneal epithelial Keratin cells. (Schermer, A, et al. (1986) J. Cell Biol., 103: 49-62).
Domaine de recherche
Structure cellulaire
Structure cellulaire
Sous-domaine de recherche
Cytokératines
Cytokératines
This Anti-Keratin K3/K76 Antibody, clone AE5 is validated for use in Western Blotting and Immunohistochemistry and Immunofluorescence for the detection of Keratin K3/K76.
Qualité
µµ
Description de la cible
Env. 60 kDa (taille observée)
Liaison
Remplace le(s) produit(s) suivant(s) : CBL218
Forme physique
Format : Produit purifié
IgG1κ monoclonale de souris purifiée, dans un tampon à base de Tris-glycine 0,1 M (pH 7,4) et de NaCl 150 mM contenant 0,05 % d'azoture de sodium.
Produit purifié sur protéine G
Stockage et stabilité
Stable entre 2 et 8 °C pendant 1 an à compter de la date de réception.
Autres remarques
Concentration : Voir la fiche technique du lot concerné.
Clause de non-responsabilité
Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Intrastromal invasion by limbal epithelial cells is mediated by epithelial-mesenchymal transition activated by air exposure.
Kawakita, T; Espana, EM; He, H; Li, W; Liu, CY; Tseng, SC
The American Journal of Pathology null
Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells.
Schermer, A; Galvin, S; Sun, TT
The Journal of cell biology null
Propagation and phenotypic preservation of rabbit limbal epithelial cells on amniotic membrane.
Wang, DY; Hsueh, YJ; Yang, VC; Chen, JK
Investigative Ophthalmology & Visual Science null
Monoclonal antibody analysis of bovine epithelial keratins. Specific pairs as defined by coexpression.
Cooper, D and Sun, T T
The Journal of Biological Chemistry, 261, 4646-4654 (1986)
Sarbani Hazra et al.
Scientific reports, 6, 21840-21840 (2016-02-26)
Successful repair of a damaged corneal surface is a great challenge and may require the use of a scaffold that supports cell growth and differentiation. Amniotic membrane is currently used for this purpose, in spite of its limitations. A thin
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