CA1023
Anti-Hsp90α Mouse mAb (EMD-17D7)
liquid, clone EMD-17D7, Calbiochem®
Synonyme(s) :
Anti-Heat Shock Protein 90α
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A propos de cet article
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
EMD-17D7, monoclonal
Species reactivity:
human
Application:
—
Citations:
12
Service technique
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Laissez-nous vous aiderbiological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
EMD-17D7, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1 µg/mL)
Immunoprecipitation (5 µg/0.7 mg protein))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Protein G PLUS/protein A purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90α protein.
Recognizes the ~90 kDa Hsp90α protein in HeLa cells. Does not recognize Hsp90β.
This Anti-Hsp90α Mouse mAb (EMD-17D7) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Hsp90α.
Immunogen
Hsp90α, His•Tag
Human
Application
ELISA (see comments)
Immunoblotting (1 g/ml)
Immunoprecipitation (5 g/0.7 mg protein)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
H460 and HeLa cells
H460 and HeLa cells
Other Notes
Detects native and recombinant Hsp90α. Does not recognize Hsp90β. This antibody will also work for ELISA, but concentration is assay dependent. Antibody should be titrated for optimal results in individual systems.
Scheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA96, 1297.
Nemoto, T., et al. 1997 J. Biol. Chem.272, 26179.
Nemoto, T., et al. 1998. Biochem. J.330, 989.
Nemoto, T., et al. 1997 J. Biol. Chem.272, 26179.
Nemoto, T., et al. 1998. Biochem. J.330, 989.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
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Classe de stockage
10 - Combustible liquids
Que se passe-t-il ?
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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