Accéder au contenu
Merck
Toutes les photos(2)

Key Documents

ABE547

Sigma-Aldrich

Anti-ALKBH5 Antibody

from rabbit, purified by affinity chromatography

Synonyme(s) :

RNA demethylase ALKBH5, Alkylated DNA repair protein alkB homolog 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

human

Réactivité de l'espèce (prédite par homologie)

bovine (based on 100% sequence homology), canine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), horse (based on 100% sequence homology)

Technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

Numéro d'accès NCBI

NP_060228.3

Numéro d'accès UniProt

Q6P6C2

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ALKBH5(54890)

Description générale

RNA demethylase ALKBH5 is also known as Alkylated DNA repair protein alkB homolog 5 and Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5. ALKBH5 is a dioxygenase that demethylates RNA, specifically N(6)-methyladenosine (m6A) RNA, which affects mRNA processing and export and is needed for spermatogenesis. ALKBH5 has the highest expression in lung, testis, pancreas, spleen and ovary, but is widely expressed across other tissues.

Immunogène

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human ALKBH5.

Application

Detect ALKBH5 using this rabbit polyclonal antibody, Anti-ALKBH5 Antibody validated for use in western blotting & IP.
Immunoprecipitation Analysis: 10 µg from a representative lot detected ALKBH5 from 500 µg of MCF-7 cell lysate.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins

Qualité

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ALKBH5 in 10 µg of MCF-7 cell lysate.

Description de la cible

~52 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Forme physique

Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

En option

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Ye Yuan et al.
Cell death & disease, 12(1), 60-60 (2021-01-13)
ALKBH5 is the main enzyme for m6A-based demethylation of RNAs and it has been implicated in many biological and pathophysiological processes. Here, we aimed to explore the potential involvement of ALKBH5 in osteosarcoma and decipher the underlying cellular/molecular mechanisms. We
RNA demethylase ALKBH5 promotes colorectal cancer progression by posttranscriptional activation of RAB5A in an m6A-YTHDF2-dependent manner.
Shen, et al.
Clinical and Translational Medicine, 13, e1279-e1279 (2023)
Zhaofeng Li et al.
Cell death & disease, 12(6), 578-578 (2021-06-06)
N6-methyladenosine (m6A) modification is widespread in messenger RNAs and increasing evidence suggests the crucial roles of m6A in cell differentiation and tissue development. However, whether m6A modulates the osteogenic differentiation of mesenchymal stem cells (MSCs) has not been fully elucidated.
Lan Hong et al.
Cancer chemotherapy and pharmacology, 92(1), 71-81 (2023-06-05)
Tumor-promotive tumor-associated macrophages (TAMs) and the CXCL16/CXCR6 axis have been reported to be correlated with the limited efficacy of chemotherapy in ovarian cancer (OC). However, the role of TAM-secreted CXCL16 and the mechanism by which it affects the cisplatin (DDP)
Wei Song et al.
Cell death discovery, 8(1), 25-25 (2022-01-16)
Long noncoding RNAs (lncRNAs) have crucial functions in the tumorigenesis and metastasis of cancers. N6-methyladenosine (m6A) modification of RNA is an important epigenetic regulatory mechanism in various malignancies. Nevertheless, the mechanism of m6A-modified lncRNA in diffuse large B cell lymphoma
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique