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Key Documents

ABE543

Sigma-Aldrich

Anti-PRDM16 Antibody

from rabbit, purified by affinity chromatography

Synonyme(s) :

PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1, MDS1/EVI1-like gene 1

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

human

Réactivité de l'espèce (prédite par homologie)

horse (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology), guinea pig (based on 100% sequence homology)

Technique(s)

immunohistochemistry: suitable ((Paraffin))
immunoprecipitation (IP): suitable
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... PRDM16(63976)

Description générale

Anti-PRDM16, Cat. No. ABE543, is a rabbit polyclonal antibody that detects PR domain zinc finger protein 16 (PRDM16) and is teste for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Target molecular weight ~140 kDa observed; 140.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogène

KLH-conjugated linear peptide corresponding to human PRDM16.

Application

Detect PRDM16 using this rabbit polyclonal antibody, Anti-PRDM16 Antibody validated for use in western blotting, IP & IHC.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated PRDM16 from 500 µg of K562 nuclear extract.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected PRDM16 in human kidney tissues.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Qualité

Evaluated by Western Blotting in LNCaP nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected PRDM16 in 10 µg of LNCaP nuclear extract.

Description de la cible

~140 kDa observed. Four isoforms are known to exist. This protein has been observed at 150 kDa, 135 kDa, and 100 kDa (EMD Millipore Cat. No. ABD130). Uncharacterized band(s) may be observed in some cell lysates.

Forme physique

Affinity purified
Purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Liang-Ru Fei et al.
Journal of experimental & clinical cancer research : CR, 38(1), 35-35 (2019-01-27)
The transcription factor PR domain containing 16 (PRDM16) is known to play a significant role in the determination and function of brown and beige fat. However, the role of PRDM16 in tumor biology has not been well addressed. Here we

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