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Key Documents

AB3712

Sigma-Aldrich

Anti-Mannosidase II Antibody, For Golgi Apparatus identification

serum, Chemicon®

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

serum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

monkey, human

Ne doit pas réagir avec

mouse

Fabricant/nom de marque

Chemicon®

Technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MAN2A1(4124)

Spécificité

Mannosidase II. This antibody is a useful marker for golgi apparatus identification.

Immunogène

Epitope: For Golgi Apparatus identification
Purified peptide from human mannosidase II.

Application

Detect the Mannosidase II protein using this Anti-Mannosidase II, For Golgi Apparatus identification validated for use in ICC & IHC.
Immunocytochemistry on HeLa and COS7 cells

Immunohistochemistry on cold methanol fixed tissue.

Optimal working dilutions must be determined by end user.
Research Category
Epitope Tags & General Use
Research Sub Category
Organelle & Cell Markers

Forme physique

Rabbit serum. Liquid with 0.02% sodium azide.

Stockage et stabilité

Maintain unopened vial at -70C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2-8C° for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8C°. For storage at -20C°, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70C° for up to 6 months. Avoid repeated freeze/thaw cycles.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Alex T-W Sin et al.
Molecular and cellular biology, 36(18), 2344-2359 (2016-06-22)
During the cell cycle, genetic materials and organelles are duplicated to ensure that there is sufficient cellular content for daughter cells. While Golgi growth in interphase has been observed in lower eukaryotes, the elaborate ribbon structure of the mammalian Golgi
Yuta Amagai et al.
Nature communications, 14(1), 2683-2683 (2023-05-10)
Many secretory enzymes acquire essential zinc ions (Zn2+) in the Golgi complex. ERp44, a chaperone operating in the early secretory pathway, also binds Zn2+ to regulate its client binding and release for the control of protein traffic and homeostasis. Notably
Azumi Yoshimura et al.
Cells, 11(1) (2022-01-12)
In the early secretory pathway, the delivery of anterograde cargoes from the endoplasmic reticulum (ER) exit sites (ERES) to the Golgi apparatus is a multi-step transport process occurring via the ER-Golgi intermediate compartment (IC, also called ERGIC). While the role
Laasya Dhandapani et al.
Journal of cell science, 135(1) (2021-12-14)
Oocytes spend the majority of their lifetime in a primordial state. The cellular and molecular biology of primordial oocytes is largely unexplored; yet, it is necessary to study them to understand the mechanisms through which oocytes maintain cellular fitness for

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