AB1032
Anti-Versican Antibody, a.a. 535-598 of mouse versican
Chemicon®, from rabbit
Synonyme(s) :
GAG alpha Domain
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
mouse
Fabricant/nom de marque
Chemicon®
Technique(s)
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... VCAN(1462)
Description générale
Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan., Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest.
Spécificité
Mouse versican GAG alpha domain
Immunogène
Epitope: a.a. 535-598 of mouse versican
GST fusion protein containing amino acids 535-598 of mouse versican.
Application
Immunohistochemistry (formalin fixed, paraffin embedded): 10 μg/mL. Tissues must be treated with Chondroitinase before staining {0.5U/mL in 100mM Tris-HCL pH 7.2-7.4, 30 minutes at room temperature} Then block and stain.
Western blotting: 0.5 μg/mL
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Western blotting: 0.5 μg/mL
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Versican Antibody, a.a. 535-598 of mouse versican is validated for use in IP, WB, IH(P) for the detection of Versican.
Forme physique
Format: Purified
Immunoglobulin was purified by affinity chromatography and is presented in PBS with no preservative Anti-GST has not been adsorbed.
Stockage et stabilité
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Remarque sur l'analyse
Control
Immunohistochemistry Positive control: surrounding cartilage, neural tube / brainWestern blotting positive control: newborn mouse brain
Immunohistochemistry Positive control: surrounding cartilage, neural tube / brainWestern blotting positive control: newborn mouse brain
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Sabrina Hsiung et al.
Scientific reports, 8(1), 7530-7530 (2018-05-16)
The mechanisms responsible for macrovascular complications in diabetes remain to be fully understood. Recent studies have identified impaired vascular repair as a possible cause of plaque vulnerability in diabetes. This notion is supported by observations of a reduced content of
Expression of a novel versican variant in dorsal root ganglia from spared nerve injury rats.
Oliver Bogen et al.
Molecular pain, 15, 1744806919874557-1744806919874557 (2019-08-21)
Kelly L Short et al.
Journal of molecular endocrinology, 64(3), 155-164 (2020-01-21)
Glucocorticoid (GC) signaling via the glucocorticoid receptor (GR) is essential for lung maturation in mammals. Previous studies using global or conditional mouse model knockouts of the GR gene have established that GR-mediated signaling in the interstitial mesenchyme of the fetal
Stephen P Evanko et al.
Methods in cell biology, 143, 261-279 (2018-01-10)
Versican is a chondroitin sulfate proteoglycan found in the extracellular matrix that is important for changes in cell phenotype associated with development and disease. Versican has been shown to be involved in cardiovascular disorders, as well as lung disease and
Sumeda Nandadasa et al.
Matrix biology plus, 10, 100064-100064 (2021-07-02)
Two inherent challenges in the mechanistic interpretation of protease-deficient phenotypes are defining the specific substrate cleavages whose reduction generates the phenotypes and determining whether the phenotypes result from loss of substrate function, substrate accumulation, or loss of a function(s) embodied
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