It comprises a 15-amino acid sequence Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser (KETAAAKFERQHMDS).
S-protein Agarose specifically retains S•Tag fusion proteins. Binding can be performed in a column or batch mode. Elution is possible using slightly denaturing conditions or using a specific protease, such as thrombin or enterokinase, with appropriate recombinant proteins. The capacity of S-protein Agarose varies and is based on the size and folding characteristics of a given target protein.
Application
S-protein Agarose has been used in the purification of recombinant S based tagged fusion proteins.[1][2][3]
Avertissement
Toxicity: Standard Handling (A)
Informations légales
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Archives of biochemistry and biophysics, 596, 63-72 (2016-03-05)
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