475904-M

Alcool polyvinylique

• Synonyme(s) : Poly(vinyl alcohol)
• Formule linéaire : [-CH₂CHOH-]_n
• Numéro CAS: 9002-89-5
• Numéro MDL: MFCD00081922
• Code UNSPSC : 41202101
• Nomenclature NACRES : NA.75

Propriétés

• Description: RTECS - TR8100000
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Couleur: yellow
• Température de stockage: 10-30°C
• InChI: 1S/C2H4O/c1-2-3/h2-3H,1H2
• Clé InChI: IMROMDMJAWUWLK-UHFFFAOYSA-N

Description

Description générale

Used for mounting cells on slides for detection of cell-surface antigens by immunofluorescence. Useful as an anti-fading agent.

Avertissement

Toxicity: Standard Handling (A)

Notes préparatoires

Add 2.4 g of Mowiol^® 4-88 to 6 g of glycerol and stir to mix. Add 6 ml of water and stir for several hours at room temperature. Add 12 ml of 0.2 M Tris (pH 8.5) and heat to 50°C for 10 min. with occasional mixing. After the Mowiol 4-88 dissolves, clarify the solution by centrifugation at 5000 x g for 15 min.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 weeks at 4°C.

Autres remarques

Longin, A., et al. 1993. J. Histochem. Cytochem. 41, 1833.
Beug, H., et al. 1979. Cell18, 375.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Mowiol is a registered trademark of Kuraray Europe GmbH

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1