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411451

Sigma-Aldrich

Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069)

liquid, clone HP6069, Calbiochem®

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.74

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

secondary antibodies

Clone

HP6069, monoclonal

Forme

liquid

Ne contient pas

preservative

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Isotype

IgG1

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Purified mouse monoclonal antibody. Recognizes human IgG1 Fc fragment.
This Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) is validated for use in ELISA for the detection of Human IgG₁, Fc Fragment Specific.

Application

ELISA (see comments and application references)

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In PBS, pH 7.4.

Reconstitution

Following initial use aliquot and freeze (-20°C).

Autres remarques

Can be used as a capture antibody for ELISA at 10 µg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG1: 100%

Human IgG2: 0.1%

Human IgG3: 0.1%

Human IgG4: <0.02%

Human IgA: <0.1%

Human IgE: <0.1%

Human IgM: <0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG1: <0.01%

Human IgG2: <0.01%

Human IgG3: <0.01%

Human IgG4: <0.01%

Human IgA: <0.01%

Human IgE: <0.001%

Human IgM: <0.01%
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

D C Houpt et al.
Infection and immunity, 62(7), 2857-2864 (1994-07-01)
Serum obtained from normal human subjects contains antibodies reactive in an enzyme-linked immunosorbent assay with the glucuronoxylomannan (GXM) of Cryptococcus neoformans. The frequency of occurrence of class-specific antibodies among normal subjects was 28% for immunoglobulin G (IgG), 98% for IgM
Elizabeth H Aitken et al.
eLife, 10 (2021-06-29)
Plasmodium falciparum causes placental malaria, which results in adverse outcomes for mother and child. P. falciparum-infected erythrocytes that express the parasite protein VAR2CSA on their surface can bind to placental chondroitin sulfate A. It has been hypothesized that naturally acquired
R G Keller et al.
Infection and immunity, 62(1), 215-220 (1994-01-01)
Previous studies found that normal human serum (NHS) contains an immunoglobulin G (IgG) antibody that mediates initiation of the classical complement pathway by nonencapsulated Cryptococcus neoformans. The present study used an enzyme-linked immunosorbent assay with whole nonencapsulated yeast cells as
H Lu et al.
Infection and immunity, 61(6), 2400-2407 (1993-06-01)
The aims of this study were to determine the immunodominant antigens of Actinobacillus actinomycetemcomitans serotype b (Aab) for the different immunoglobulin (Ig) classes and subclasses and to determine the relative levels of these different Igs in serum. Seropositive early-onset periodontitis

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