345797

α1-3,6-Galactosidase, Recombinant, E. coli

Catalyzes the hydrolysis of α1,3-linked and α1,6-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins.

• Synonyme(s) : α1-3,6-Galactosidase, Recombinant, E. coli, α1-3,6-D-Galactoside Galactohydrolase
• Numéro CAS: 9025-35-8
• Numéro CE : 3.2.1.22 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54
• MDL number: MFCD00130486
• Specific activity: ≥30 units/mg protein, ≥85 units/mL
• Recombinant: expressed in E. coli

Propriétés

• recombinant: expressed in E. coli
• Quality Segment: 100
• form: liquid
• specific activity: ≥30 units/mg protein, ≥85 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• foreign activity: Proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Recombinant α1-3,6-galactosidase expressed in E. coli. Catalyzes the hydrolysis of α1,3-linked and α1,6-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. Particularly efficient in removing α-linked galactose under conditions where the pH must be neutral or above, as with live cells.

Physical form

In 50 mM sodium phosphate buffer, pH 7.5.

Other Notes

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-α-D-galactopyranoside per min at 25°C, pH 6.5.

Prime, S., et al. 1996. J. Chromatogr. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Informations sur la sécurité

• Classe de stockage: 10 - Combustible liquids
• wgk: WGK 2