09-818

Anti-CARM1 Antibody

from rabbit

• Synonyme(s) : Protein arginine N-methyltransferase 4, coactivator-associated arginine methyltransferase 1, coactivator-associated arginine methyltransferase-1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 18

Propriétés

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, human
• species reactivity (predicted by homology): mouse
• technique(s): western blot: suitable
• NCBI accession no.: NM_199141.1
• UniProt accession no.: Q9WVG6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CARM1(10498) ; mouse ... Carm1(59035)

Description

General description

CARM1 (coactivator-associated arginine methyltransferase 1) is an arginine N-methyltransferase that catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to arginine residues within proteins. CARM1 targets include methylation of histone H3 at Arg17 and p300 at Arg2142. CARM1 is recruited to promoters upon gene activation together with histone acetyltransferases from the p300 and p160 families. During monocyte inflammatory stimulation, CARM1 acts together with p300 as a coactivator for NF-kB. It is responsible for moving cells toward the inner cell mass in developing blastocysts. CARM1 also plays an important role in androgen receptors and may play a role in prostate cancer progression.

~60 kDa

Immunogen

Epitope: a.a. 595-608

KLH-conjugated, synthetic peptide containing the sequence (CSPMSIPTNTMHYGS), corresponding to a.a. 595-608 of human CARM1.

Application

Anti-CARM1 Antibody is a rabbit polyclonal antibody for detection of CARM1 also known as Protein arginine N-methyltransferase 4, coactivator-associated arginine methyltransferase 1 & has been validated in WB.

Western Blot Analysis:
A 1 μg/mL dilution of this lot detected CARM1 in HeLa nuclear extracts. (Figure A).

Biochem/physiol Actions

Recognizes CARM1, Mr ~60 kDa.

Physical form

Format: Purified

Purified by peptide affinity chromatography. Supplied at 1 mg/ml in 0.1 M Tris-Glycine (pH7.4) 15 mM NaCl, 0.05% sodium azide.

Analysis Note

Control
Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations sur la sécurité

• Classe de stockage: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable