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Key Documents

05-666

Sigma-Aldrich

Anti-BRCA2 Antibody, clone 5.23

clone 5.23, Upstate®, from mouse

Synonyme(s) :

Anti BRCC2, Anti-BROVCA2, Anti-FACD, Anti-FAD, Anti-FAD1, Anti-FANCD, Anti-FANCD1, Anti-GLM3, Anti-PNCA2, Anti-XRCC11

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

5.23, monoclonal

Espèces réactives

human

Fabricant/nom de marque

Upstate®

Technique(s)

immunocytochemistry: suitable
western blot: suitable

Isotype

IgG

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... BRCA2(675)

Spécificité

BRCA2

Immunogène

GST fusion protein to residues 2959-3418 of human BRCA2 protein

Application

Detect BRCA2 with Anti-BRCA2 Antibody, clone 5.23 (Mouse Monoclonal Antibody), that has been shown to work in WB, ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualité

routinely evaluated by immunoblot on in HeLa nuclear extract

Description de la cible

390kDa

Forme physique

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 0.05% sodium azide.
Format: Purified
Protein G Chromatography

Stockage et stabilité

Store at 2-8°C. Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C.

Remarque sur l'analyse

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Autres remarques

Due to license agreement restrictions, this product cannot be purchased for resale.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1


Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Loredana Moro et al.
The Journal of biological chemistry, 280(23), 22482-22491 (2005-04-05)
BRCA2 is a tumor suppressor gene that when mutated confers an increased susceptibility to developing breast and prostate carcinoma. Besides its role in mediating DNA repair, new evidence suggests that BRCA2 may also play a role in suppressing cancer cell
Jimmy M-M Kwok et al.
Molecular cancer research : MCR, 8(1), 24-34 (2010-01-14)
The transcription factor Forkhead box M1 (FOXM1) is a key regulator of cell proliferation and is overexpressed in many forms of primary cancers, leading to uncontrolled cell division and genomic instability. To address the role of FOXM1 in chemoresistance, we
Loredana Moro et al.
The Journal of biological chemistry, 281(31), 22100-22107 (2006-06-07)
Aberrant interaction of carcinoma cells with basement membranes (BM) is a fundamental pathophysiological process that initiates a series of events resulting in cancer cell invasion and metastasis. In this report, we describe the results of our investigations pertaining to the
Yongjun Tan et al.
Molecular and cellular biology, 27(3), 1007-1016 (2006-11-15)
The forkhead box M1 (FoxM1) transcription factor regulates expression of cell cycle genes essential for DNA replication and mitosis during organ repair and cancer progression. Here, we demonstrate that FoxM1-deficient (-/-) mouse embryonic fibroblasts and osteosarcoma U2OS cells depleted in
Miyoung Lee et al.
Nucleic acids research, 49(10), 5588-5604 (2021-05-13)
Cancer-causing missense mutations in the 3418 amino acid BRCA2 breast and ovarian cancer suppressor protein frequently affect a short (∼340 residue) segment in its carboxyl-terminal domain (DBD). Here, we identify a shared molecular mechanism underlying their pathogenicity. Pathogenic BRCA2 missense

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