04-824
Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal
culture supernatant, clone 68-4, from rabbit
Synonyme(s) :
Estradiol receptor, Nuclear receptor subfamily, estrogen receptor 1, estrogen receptor alpha
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
culture supernatant
Type de produit anticorps
primary antibodies
Clone
68-4, monoclonal
Espèces réactives
mouse, rat, human
Technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... ESR2(2100)
mouse ... Esr2(13983)
rat ... Esr2(25149)
Description générale
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.
Spécificité
Estrogen receptor β, long form, does not detect estrogen receptor alpha.
Predicted to cross-react with mouse and rat based on sequence homology.
Immunogène
Epitope: a.a.55-74(H) or 63-82 (R, M)
KLH-conjugated, synthetic peptide corresponding to amino acids 63-82 of rat and mouse estrogen receptor β and amino acids 55-74 of human estrogen receptor β.
Application
Detect Estrogen Receptor β using this Anti-Estrogen Receptor β Antibody, clone 68-4 validated for use in WB & IP.
Immunoprecipitation:
10 μL of a previous lot immunoprecipitated estrogen receptor β from 500 μg of MCF7 RIPA lysate.
10 μL of a previous lot immunoprecipitated estrogen receptor β from 500 μg of MCF7 RIPA lysate.
Qualité
Evaluated by western blot on RIPA lysates from MCF-7 cells.
Western Blot Analysis:
A 1:500 to 1:2,000 dilution of this antibody detected estrogen receptor β in RIPA lysates from MCF-7 cells.
Western Blot Analysis:
A 1:500 to 1:2,000 dilution of this antibody detected estrogen receptor β in RIPA lysates from MCF-7 cells.
Description de la cible
59kDa
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Cheng Zeng et al.
Reproduction (Cambridge, England), 160(3), 481-490 (2020-06-17)
Endometriosis is an estrogen-dependent disease, and estrogen receptor 2 (ESR2) plays a critical role in the pathogenesis of ovarian endometriosis by promoting cell invasion. Yes-associated protein 1 (YAP1) plays suppressive roles in several types of tumors. However, the relationship between
XiaoQing Wang et al.
Frontiers in endocrinology, 12, 666195-666195 (2021-09-18)
Endometriosis is an estrogen-dependent chronic disease. The abnormal proliferation and invasion of ectopic stromal cells (ESCs) are important manifestations of endometriosis, and it is necessary to find safer and more effective treatments. Extracellular vesicles (EVs) derived from human umbilical cord
Anna Karpeta et al.
Journal of applied toxicology : JAT, 36(12), 1558-1567 (2016-03-19)
Data concerning the possible action of polybrominated diphenyl ethers (PBDEs) in hormone-dependent cancer are scarce. Some data showed that PBDEs may directly affect breast cancer cells formation and only one research showed increased proliferation of the OVCAR-3 cells, but the
F de C Gomes et al.
Andrology, 8(1), 211-220 (2019-04-24)
Nandrolone decanoate (ND) is an anabolic-androgenic steroid, and its indiscriminate use leads to subclinical alterations in the hypothalamic-pituitary-gonadal axis and androgen-dependent organs. To evaluate the effects of ND, either alone or in combination with resistance exercise (RE), on the levels
Katharina Röck et al.
The Journal of biological chemistry, 287(24), 20056-20069 (2012-04-12)
Hyaluronan (HA) and versican are key components of the dermis and are responsive to ultraviolet (UV)B-induced remodeling. The aim of this study was to explore the molecular mechanisms mediating the effects of estrogen (E(2)) on HA-rich extracellular matrix during photoaging.
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