04-773
Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal
culture supernatant, clone SA19, from rabbit
Synonyme(s) :
Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, myosin phosphatase, target subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12A
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A propos de cet article
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
SA19, monoclonal
Species reactivity:
rat, chicken, mouse, human
Application:
WB
Citations:
5
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rabbit
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
SA19, monoclonal
species reactivity
rat, chicken, mouse, human
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pThr850)
Gene Information
human ... PPP1R12A(4659)
General description
Doublet at ~130 kDa
Phosphorylation of myosin is crucial to smooth muscle contraction. The degree of myosin phosphorylation is dependent in part by the activity of myosin phosphatase. Recent findings suggest that this enzyme can be regulated, which has lead to interest in characterizing this phosphatase. Myosin phosphatase seems to be comprised of three subunits (catalytic subunit of type 1 phosphatase-δ (PP1) and two non-catalytic subunits, large and small (M20)). Studies suggest that the large subunit is a targeting subunit and is termed myosin phosphatase target subunit (MYPT). MYPT has been shown to be a substrate for the Rho-associated kinase (ROK). ROK mediates an agonist-induced increase in myosin phosphorylation and force by inhibiting myosin light chain phosphatase activity through phosphorylation of MYPT.
Immunogen
Epitope: pThr850(Ch) or pThr853(H)
KLH-conjugated, synthetic peptide containing the sequence …RS[pT]GV…, in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.
Application
Anti-phospho-MYPT1 (Thr850) Antibody, detects level of phospho-MYPT1 (Thr850) & has been published & validated for use in WB.
Biochem/physiol Actions
Predicted to cross-react with mouse and rat.
Recognizes MYPT1 phosphorylated on Thr850, doublet at Mr ~130 kDa. A non-specific protein was also detected, Mr ~55-60 kDa.
Analysis Note
Control
HEK293 cell lysate phosphorylated by ROKα/ROCKII.
HEK293 cell lysate phosphorylated by ROKα/ROCKII.
Westerin Blot Analysis: A 1:500-1:1000 dilution of this antibody detected MYPT1 in RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 05-773
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Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificats d'analyse (COA)
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Ebbe Boedtkjer et al.
Circulation, 124(17), 1819-1829 (2011-09-29)
Disturbances in pH affect artery function, but the mechanistic background remains controversial. We investigated whether Na(+), HCO₃- transporter NBCn1, by regulating intracellular pH(pH₁), influences artery function and blood pressure regulation. Knockout of NBCn1 in mice eliminated Na+, HCO₃⁻ cotransport and
E V Bouzinova et al.
Acta physiologica (Oxford, England), 224(1), e13059-e13059 (2018-02-27)
The Na,K-ATPase is involved in a large number of regulatory activities including cSrc-dependent signalling. Upon inhibition of the Na,K-ATPase with ouabain, cSrc activation is shown to occur in many cell types. This study tests the hypothesis that acute potentiation of
Vincent Sauzeau et al.
Methods in molecular biology (Clifton, N.J.), 1527, 213-218 (2017-01-25)
Increased arterial tone and the resulting rise in peripheral vascular resistance are major determinants of the elevated arterial pressure in hypertension. The RhoA/Rho kinase signaling pathways are now recognized as a major regulator of vascular smooth muscle contraction and arterial
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| 04-773 | 04053252326356 |