TP0200
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification
sufficient for ~50 manual assays
Synonym(s):
Total Protein Assay Kit
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Product Name
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification, sufficient for ~50 manual assays
description
wavelength 500-750nm
usage
sufficient for ~50 manual assays
protein detection level measuring range
0-1 mg/mL
parameter
18-26 °C temperature
200 μL sample volume
storage temp.
2-8°C
Quality Level
Application
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:
- in the dialyzates/extracts of gliadins
- in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoa, Salvia hispanica L.)
- in the stored samples of rat liver microsomal fractions
Biochem/physiol Actions
Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.
General description
The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.
Kit Components Also Available Separately
Product No.
Description
SDS
- B3934Biuret reagent, protein detection level 150-1,000 μg/mL 110 mL
- Folin & Ciocalteu’s phenol reagent 5 mL
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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